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GABA通过抑制内质网未折叠蛋白反应对抗酒精的肝毒性作用

发表时间:2016-12-15  浏览量:1451  下载量:296
全部作者: 刘艳丽,罗丹,李韬,王双连
作者单位: 山东省肿瘤医院基础研究中心;山东大学医学院
摘 要: 研究采用噻唑蓝(MTT)实验和Western blotting等方法,探讨了伽马氨基丁酸(gamma-aminobutyric acid,GABA)是否通过内质网(endoplasmic reticulum,ER)未折叠蛋白反应(unfolded protein response,UPR)发挥对抗酒精对肝细胞的增殖抑制作用。结果发现,酒精对体外培养的BRL肝细胞的增殖抑制作用可被GABA逆转,且酒精诱导的UPR标志物GRP78和CHOP表达上调被GABA抑制。以上结果说明GABA抑制酒精的肝毒性作用与GABA抑制酒精诱导的UPR有关。
关 键 词: 人体生理学;肝脏生理;酒精性肝病;伽马氨基丁酸;未折叠蛋白反应;增殖
Title: Protectiverole of GABA against ethanol hepatotoxicity involves inhibition of endoplasmic reticulum unfolded protein response
Author: LIU Yanli, LUO Dan, LI Tao, WANG Shuanglian
Organization: Basic Research Center, Shandong Tumor Hospital; School of Medicine, Shandong University
Abstract: Using MTT and Western blotting analysis, whether unfolded protein response (UPR) of endoplasmic reticulum (ER) involves in the cytoprotective effect of gamma-aminobutyric acid (GABA) against ethanol-induced hepatotoxicity was investigated. The results showed that ethanol-induced decrease of cell viability was inhibited by GABA treatmentin cultured BRL hepatocytes. GABA inhibited ethanol-induced up-regulated expression of GRP78 and CHOP, which are UPR markers. These results suggest that inhibition of UPR is involved in the protective role of GABA against ethanol hepatotoxicity.
Key words: human physiology; liver physiology; alcoholic liver disease; gamma-aminobutyric acid; unfolded protein response; proliferation
发表期数: 2016年12月第23期
引用格式: 刘艳丽,罗丹,李韬,等. GABA通过抑制内质网未折叠蛋白反应对抗酒精的肝毒性作用[J]. 中国科技论文在线精品论文,2016,9(23):2445-2449.
 
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