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调节性B细胞对矽肺小鼠纤维化Th免疫应答的影响

发表时间:2016-12-15  浏览量:1148  下载量:415
全部作者: 刘芳炜,李超,陈杰
作者单位: 中国医科大学公共卫生学院
摘 要: 目的:研究调节性B细胞(regulatory B cells,Bregs)对矽肺纤维化Th免疫应答的影响。方法:健康成年雌性C57BL/6小鼠,按体重排序后随机分为对照组、矽肺组和Bregs消除组。矽肺组和Bregs消除组小鼠行非暴露式气管内分别灌注50 μL二氧化硅悬液(3 g/50 mL)。Bregs消除组小鼠腹腔注射300 μg anti-CD22 mAb单克隆抗体以消除小鼠体内Bregs. 分别于灌注后7,28,56 d处死小鼠,收集肺组织及肺泡灌洗液上清(broncho alveolar lavage fluid,BALF)。测定肺组织羟脯氨酸含量。采用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测BALF中的细胞因子白介素-2(interleukin-2,IL-2)和IL-13. 结果:二氧化硅灌注后56 d,Bregs消除组小鼠肺组织羟脯氨酸含量显著低于矽肺组小鼠(P<0.05)。二氧化硅灌注后28 d,Bregs消除组小鼠IL-2的分泌水平显著高于对照组小鼠及矽肺组小鼠(P<0.05)。二氧化硅灌注后56 d,Bregs消除组小鼠IL-13的分泌水平显著低于矽肺组小鼠(P<0.05)。结论:Bregs可通过影响Th1/Th2免疫平衡促进矽肺小鼠胶原沉积。
关 键 词: 劳动卫生学;公共卫生;矽肺;调节性B细胞;Th应答
Title: Effect of regulatory B cells on Th immune responses in fibrosis lung of silicosis mice
Author: LIU Fangwei, LI Chao, CHEN Jie
Organization: School of Public Health, China Medical University
Abstract: Objective: To observe the effect of regulatory B cells (Bregs) on Th immune responses in silica-induced lung fibrosis in mice. Methods: Healthy adult female C57BL/6 mice were divided into three groups: control group, silicosis group and Bregs depletion group. To set up the silicosis model, mice in silicosis group and Bregs depletion group received 50 μL of silica suspension (3 g/50 mL) intratracheally. To deplete Bregs, mice in Bregs depletion group were injected intraperitoneally with 300 μg anti-CD22 mAb one day before the silica exposure. Mice were sacrificed at 7, 28, 56 days after silica exposure. Broncho alveolar lavage fluid (BALF) and lung tissues were obtained. The hydroxyproline content in mice lungs was obtained and the secretions of cytokines interleukin-2 (IL-2) and IL-13 were examined by enzyme linked immunosorbent assay (ELISA). Results: The hydroxyproline content of lung in Bregs depletion group decreased obviously at day 56 after silica exposure compared with that in silicosis group (P<0.05). The secretion of IL-2 in Bregs depletion group increased significantly at day 28 after silica exposure compared with that in control group and silicosis group (P<0.05). The secretion of IL-13 in Bregs depletion group decreased significantly at day 56 after silica exposure compared with that in silicosis group (P<0.05). Conclusion: Bregs promoted the collagen deposition in mice lung by affecting Th1/Th2 immune balance.
Key words: labour hygiene; public health; silicosis; regulatory B cells; Th responses
发表期数: 2016年12月第23期
引用格式: 刘芳炜,李超,陈杰. 调节性B细胞对矽肺小鼠纤维化Th免疫应答的影响[J]. 中国科技论文在线精品论文,2016,9(23):2465-2469.
 
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