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超高效液相色谱串联三重四极杆质谱法同时测定银黄颗粒中16种成分含量

发表时间:2016-12-15  浏览量:1496  下载量:247
全部作者: 邰艳妮,曾繁天,周小红,向青,许文,黄鸣清
作者单位: 福建中医药大学药学院
摘 要: 建立超高效液相色谱串联三重四极杆质谱(ultra-high performance liquid chromatography-tandem triple quadruple mass spectrometry,UPLC-QqQ-MS/MS)法快速定量测定银黄颗粒中16种成分(新绿原酸、绿原酸、咖啡酸、隐绿原酸、芦丁、野黄芩苷、木犀草苷、异绿原酸B、异绿原酸A、异绿原酸C、黄芩苷、汉黄芩苷、木犀草素、黄芩素、白杨素、千层纸素A)的含量。采用UPLC-QqQ-MS/MS法,并使用负离子多反应监测(multiple reaction monitoring,MRM)模式进行含量测定。色谱条件:采用Waters CORTECS C18(2.1 mm×100 mm,1.6 μm)色谱柱,以乙腈(A)-0.1%甲酸水(B)为流动相进行梯度洗脱,流速0.25 mL/min,柱温45℃. 16种成分在所考察浓度范围内线性关系良好(R>0.995 2),回收率和相对标准偏差(relative standard deviation,RSD)分别在98.67%~100.76%和2.7%~4.5%范围内。本研究所采用的UPLC-QqQ-MS/MS定量分析方法简便、快速、分离效果好,可作为银黄颗粒多成分定量评价的测定方法,为全面控制银黄颗粒的质量提供新的技术手段。
关 键 词: 中药学;银黄颗粒;UPLC-MS/MS;含量测定
Title: Simultaneous determination of 16 components in Yinhuang granules by UPLC-QqQ-MS/MS
Author: TAI Yanni, ZENG Fantian, ZHOU Xiaohong, XIANG Qing, XU Wen, HUANG Mingqing
Organization: School of Pharmacy, Fujian University of Traditional Chinese Medicine
Abstract: To use a rapid and accurate ultra-high performance liquid chromatography-tandem triple quadruple mass spectrometry (UPLC-QqQ-MS/MS) method for the determination of 16 main components (Neochlorogenic acid, Chlorogenic acid, Caffeic acid, Cryptochlorogenic acid, Rutin, Scutellarin, Cynaroside, Isochlorogenic acid B, Isochlorogenic acid A, Isochlorogenic acid C, Baicalin, Wogonoside, Luteolin, Baicalein, Chrysin, and Oroxylin A) in Yinhuang granules. The UPLC-QqQ-MS/MS assay was performed on a Waters CORTECS C18 (2.1 mm×100 mm, 1.6 μm) column with acetonitrile-water (containing 0.1% formic acid) as the mobile phase by gradient elution at a flow rate of 0.25 mL/min; The column temperature was set as 45℃. MS detection was performed with multiple reaction monitoring (MRM) mode using negative electrospray ionization. Satisfactory linearity was achieved within the investigated linear range and with fine determination coefficient (R>0.995 2), the overall recoveries were ranged from 98.67% to 100.76% with the relative standard deviation (RSD) ranging from 2.7% to 4.5%. It is the first report of simultaneous analysis of 16 major components in Yinhuang granules by using UPLC-QqQ-MS/MS method, which affords a highly sentive, specific, speedy and efficient method for controling the quality of Yinhuang granules.
Key words: traditional Chinese medicine; Yinhuang granules; UPLC-QqQ-MS/MS; quantitative determination
发表期数: 2016年12月第23期
引用格式: 邰艳妮,曾繁天,周小红,等. 超高效液相色谱串联三重四极杆质谱法同时测定银黄颗粒中16种成分含量[J]. 中国科技论文在线精品论文,2016,9(23):2470-2478.
 
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