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生物素和乳糖酸修饰纳米粒子载槲皮素治疗肝肿瘤研究

发表时间:2016-12-31  浏览量:1850  下载量:433
全部作者: 高秀军,吴立佳,司佳,刘增泉,柴强,刘冰艳,胡雪
作者单位: 天津医科大学生物医学工程与技术学院
摘 要: 合成靶向材料生物素-聚乙二醇-聚(乳酸-共聚-羟基乙酸)-聚乙二醇-生物素(BPPPB)、乳糖酸-聚乙二醇-聚(乳酸-共聚-羟基乙酸)-聚乙二醇-乳糖酸(LPPPL);构建包载槲皮素肝肿瘤双靶头载体。核磁氢谱(1H nuclear magnetic resonance,1H NMR)表征材料结构;透射电镜(transmission electron microscope,TEM)、动态激光光散射(dynamic laser light scattering,DLS)表征载体形貌和粒径;细胞体外靶向性实验表征载体靶向能力;体外细胞毒性实验(MTT实验)和急性毒性实验(昆明小鼠)表征载体的生物相容性。1H NMR结果证明材料成功合成;TEM和DLS结果表明载体表面形貌呈球形,分散均匀;细胞毒性和荧光显微镜实验表明,双靶头载体对Hep G2细胞的靶向能力最强;MTT实验和急性毒性实验(昆明小鼠)表明载体有良好的生物相容性。载体在治疗肝肿瘤领域具有较好的应用潜景。
关 键 词: 功能高分子材料;双靶头;靶向;槲皮素;聚(乳酸-共聚-羟基乙酸)
Title: Quercetin-loaded biotin and lactic acid targeting nanoparticles for hepatocellular carcinoma therapy
Author: GAO Xiujun, WU Lijia, SI Jia, LIU Zengquan, CHAI Qiang, LIU Bingyan, HU Xue
Organization: School of Biomedical Engineering and Technology, Tianjin Medical University
Abstract: Dual targeting materials biotin-poly(ethylene glycol)-poly(lactic-co-glycolid acid)-poly(ethylene glycol)-biotin (BPPPB) and LAC-poly(ethylene glycol)-poly(lactic-co-glycolid acid)-poly(ethylene glycol)-LAC (LPPPL) were synthesized and quercetin-loaded nanoparticle was constructed by nanoprecipitation. The structure of the material was showed by 1H nuclear magnetic resonance (1H NMR). The morphology and size were measured by transmission electron microscope (TEM) and dynamic laser light scattering (DLS), respectively. The targeting ability was characterized by in vitro cell-targeting assay. The biocompatibility was testified by in vitro cytotoxicity-test (MTT assay) and acute toxicity assay (Kunming mice). The results of 1H NMR showed that BPPPB and LPPPL were synthesized successfully. The results of TEM and DLS showed that nanoparticles were spherical appearance and had small size distribution. The result of fluorescence microscope showed that the targeting ability of BPPPB nanoparticles to Hep G2 cells was stronger than that of HL-7702 cells. MTT assay and acute toxicity assay (Kunming mice) were used to prove the biocompatibility of nanoparticles. Those results showed that the dual targeting nanoparticles had a good application potential in drug delivery system.
Key words: functional polymer materials; dual targeting groups; target; quercetin; poly(lactic-co-glycolid acid)
发表期数: 2016年12月第24期
引用格式: 高秀军,吴立佳,司佳,等. 生物素和乳糖酸修饰纳米粒子载槲皮素治疗肝肿瘤研究[J]. 中国科技论文在线精品论文,2016,9(24):2510-2518.
 
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