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拟南芥花粉发育磷酸化蛋白质组学研究

发表时间:2017-02-16  浏览量:1311  下载量:243
全部作者: 陈艳梅
作者单位: 中国农业大学生物学院
摘 要: 在高等植物有性生殖过程中,花粉管作为运输精子到胚珠的载体,其生长具有高度极性化。由于花粉管本身所具有的特性,它已经成为研究细胞相互识别、胞内和胞外信号的模式系统。为研究蛋白磷酸化在拟南芥花粉管生长中的作用,使用定量蛋白质组学手段,分析花粉发育过程中磷酸化蛋白变化图谱。通过质谱分离鉴定到44个磷酸化蛋白, 定量分析发现,花粉萌发后,25个蛋白磷酸化程度增加,19个蛋白磷酸化程度降低。根据其生物学功能,这些蛋白可分为信号转导、小泡运输、细胞骨架和碳水化合物代谢等几类。研究发现,花粉管生长过程能量代谢水平增加到非常活跃的状态,此外,在花粉发育过程中,磷酸化在细胞分泌途径中起重要作用。
关 键 词: 植物学;花粉;蛋白磷酸化;膜运输;细胞骨架
Title: Phosphoproteomic analysis of Arabidopsis pollen development
Author: CHEN Yanmei
Organization: College of Biological Sciences, China Agricultural University
Abstract: Pollen tube is a carrier to deliver the sperm to the ovule during the process of sexual plant reproduction in higher plants. Pollen tube growth is highly polarized and has become a relatively well-characterized model system in which to study cell-cell recognition, intra- and intercellular signaling in higher plants. To investigate the roles of the protein phosphorylation in the growth of pollen tube of Arabidopsis thaliana, a quantitative approach was used to analyze the protein phosphorylation dynamic changes during pollen development. A total of 44 phosphoproteins were reproducibly identified in this study, among the identified proteins, in case of 25 proteins were increased on the phosphorylation level while 19 proteins were decreased after pollen germination. These proteins were grouped into distinct functional categories including proteins involved in signaling transduction, membrane trafficking, cytoskeleton and carbohydrate metabolism. These data indicated that energy metabolism was increased to an active level, furthermore, protein phosphorylation played a crucial role in the regulation of membrane trafficking pathways in growing pollen tubes.
Key words: botany; pollen; protein phosphorylation; membrane trafficking; cytoskeleton
发表期数: 2017年2月第3期
引用格式: 陈艳梅. 拟南芥花粉发育磷酸化蛋白质组学研究[J]. 中国科技论文在线精品论文,2017,10(3):265-270.
 
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