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菊花DgCCD7启动子克隆与顺式作用元件分析

发表时间:2017-02-16  浏览量:1679  下载量:393
全部作者: 郗 琳,温 超,吕素慧,聂 静,杨云燕,赵梁军
作者单位: 中国农业大学园艺学院,花卉发育与品质调控北京市重点实验室
摘 要: 以切花菊‘神马’(Dendranthema grandiflorum cv.‘Jinba’)为材料,对菊花独脚金内酯(strigolactone,SL)合成基因DgCCD7基因启动子克隆,获得DgCCD7基因启动子1 442 bp. 利用数据库PLACE分别对菊花SL合成基因DgCCD7与DgCCD8的启动子序列进行分析,并对两者上游1 200 bp内的顺势作用元件进行比对分析,发现2个基因均含有2个共同的生长素相关元件:生长素响应元件CATATGGMSAUR以及类生长素响应元件SEBFCONSSTPR10A;除此之外,在2个启动子共同含有的元件中,均具有一个P1Bs结合位点,分别位于ProDgCCD7的-610 bp和ProDgCCD8的-427 bp,该位点参与响应磷胁迫相关转录因子PHR1的结合。通过转染拟南芥GUS染色结果也可看到DgCCD7在下胚轴及分生组织染色较深,其根部的GUS表达受到NAA与缺磷的诱导。研究结果为进一步研究基因DgCCD7与DgCCD8的转录调控机制奠定基础,以期揭示SL对菊花侧枝发育的调控机理,为进一步利用人工合成的SL调控菊花侧枝生长特性提供理论和实践基础。
关 键 词: 园艺学;菊花;启动子;CCDs;独脚金内酯;转录调控
Title: Clone and cis-element analysis of DgCCD7 promoter in chrysanthemums
Author: XI Lin, WEN Chao, LÜ Suhui, NIE Jing, YANG Yunyan, ZHAO Liangjun
Organization: Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, College of Horticulture, China Agricultural University
Abstract: Strigolactone (SL) is known as a long-distance signal in regulation of chrysanthemum shoot branching. The promoter of SL biosynthesis gene DgCCD7 was isolated from chrysanthemum ‘Shenma’ (Dendranthema grandiflorum cv. ‘Jinba’), which contains full-length of 1 442 bp. By using PLACE database, analysis of promoter sequences of DgCCD7 and DgCCD8 as well as comparative analysis of the cis-regulatory elements within the upstream of 1 200 bp were carried out. Two common auxin related components were found in both genes, which are auxin responsive motif CATATGGMSAUR and auxin-like responsive motif SEBFCONSSTPR10A. Furthermore, one P1Bs binding motif were identified on both DgCCD7 and DgCCD8 promoters, which located on -610 bp of ProDgCCD7 and -427 bp of ProDgCCD8, respectively. And this binding motif is involved in the binding of phosphorus stress related transcription factor PHR1. By transformation of Arabidopsis fusion with GUS gene, DgCCD7 promoter can be activated at meristems and hypocotyledonary region. Meanwhile NAA and Pi starvation can active GUS expression in roots of transgene Arabidopsis. To sum up, this study may lay a foundation on the study of the transcriptional regulation mechanism of gene DgCCD7 and DgCCD8 to reveal the regulation mechanism of SL on shoot branching of chrysanthemum, which can be used for controlling branching during cut flower production.
Key words: horticulture; chrysanthemum; promoter; CCDs; strigolactone; transcription regulation
发表期数: 2017年2月第3期
引用格式: 郗 琳,温 超,吕素慧,等. 菊花DgCCD7启动子克隆与顺式作用元件分析[J]. 中国科技论文在线精品论文,2017,10(3):281-290.
 
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2017-02-22 10:29:37
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