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pM蛋白与IgY的互作研究

发表时间:2017-02-16  浏览量:1149  下载量:358
全部作者: 江雪梅,张小莺
作者单位: 西北农林科技大学动物医学院
摘 要: 目的:验证pM蛋白与卵黄抗体(IgY)的结合关系,并探明其亲和力大小。方法:使用Pull down、Western blotting法、酶解法以及表面等离子共振(surface plasmon resonance,SPR)的方法探索pM蛋白与IgY的结合机制。结果:pM蛋白与鸡、鸭IgY存在较强的结合关系,并且pM蛋白与IgY的结合主要依赖于IgY的Fab区域。结论:pM蛋白能够作为IgY纯化的配体使用。
关 键 词: 兽医学;卵黄抗体IgY;pM蛋白;互作研究
Title: Interaction study of protein pM and IgY
Author: JIANG Xuemei, ZHANG Xiaoying
Organization: College of Veterinary Medicine, Northwest A & F University
Abstract: Objective: To verify the binding relationship between protein pM and egg yolk immunoglobulin (IgY) and confirm its affinity. Methods: Pull down assay, Western blotting, enzyme hydrolysis method and surface plasmon resonance (SPR) were used to conduct the binding mechanism of pM and IgY. Results: These results confirmed protein pM can bind with chicken IgY, as well as IgY from Anatidae birds, by blocking the variable region Fab of IgY. Conclusion: Protein pM can be used as IgY purified ligand.
Key words: veterinary science; egg yolk immunoglobulin (IgY); protein pM; interaction study
发表期数: 2017年2月第3期
引用格式: 江雪梅,张小莺. pM蛋白与IgY的互作研究[J]. 中国科技论文在线精品论文,2017,10(3):341-345.
 
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