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海洋链霉菌LS298次级代谢产物研究

发表时间:2017-03-15  浏览量:1091  下载量:304
全部作者: 甄心,巩婷,朱平
作者单位: 中国医学科学院&北京协和医学院药物研究所,天然药物活性物质与功能国家重点实验室,国家卫生和计划生育委员会天然药物生物合成重点实验室
摘 要: 目的:对海洋链霉菌LS298的微量次级代谢产物进行研究。方法:采用Sephadex LH-20凝胶柱色谱、高效液相色谱(high performance liquid chromatography,HPLC)等方法对LS298发酵产物进行分离纯化;通过核磁共振(nuclear magnetic resonance,NMR)、电喷雾电离质谱(electrospay ionization mass spectrometer,ESI-MS)等波谱分析手段对分离得到的化合物进行结构鉴定。结果:分离得到6个化合物,包括3个星孢霉素类化合物,4'-N-甲基星孢霉素(1)、7-羟基星孢霉素(2)和7-去氢星孢霉素(3);3个其他类化合物,光黄素(4)、N-乙酰基-β-酮-色胺(5)和尼克酰胺(6)。结论:LS298菌株的次级代谢产物表现出结构类型的多样性,这也是本菌株的特点。
关 键 词: 药物化学;链霉菌;次级代谢产物;星孢霉素;结构鉴定
Title: Study on secondary metabolites produced by marine Streptomyces sp. LS298
Author: ZHEN Xin, GONG Ting, ZHU Ping
Organization: Key Laboratory of Biosynthesis of Natural Products of the National Health and Family Planning Commission, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College
Abstract: Objective: To study the trace secondary metabolites produced by the marine Streptomyces sp. LS298. Methods: The isolation and purification of the metabolites from the fermentation broth were conducted by Sephadex LH-20 gel column chromatography, high performance liquid chromatography (HPLC) and other chromatographic separation process; the nuclear magnetic resonance (NMR), electrospay ionization mass spectrometer (ESI-MS) and other spectroscopic methods were applied in the structural identification of the purified compounds. Results: Six compounds were purified and identified, three of which were analogues of staurosporine: 4'-N-methyl-staurosporine (1), 7-hydroxyl-staurosporine (2), 7-dehydro-staurosporine (3); and the other three compounds were lumichrome (4), N-acetyl-β-oxotryptamine (5), and niacinamide (6). Conclusion: The secondary metabolites of LS298 were rich in the structural types, and structural diversities were the characteristics of the strain.
Key words: medicinal chemistry; Streptomyces; secondary metabolites; staurosporine; structural identification
发表期数: 2017年3月第5期
引用格式: 甄心,巩婷,朱平. 海洋链霉菌LS298次级代谢产物研究[J]. 中国科技论文在线精品论文,2017,10(5):534-539.
 
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