您的位置:首页  > 论文页面

茉莉酸甲酯对杠柳不定根中次生产物的诱导和生理机制研究

发表时间:2017-03-15  浏览量:1111  下载量:172
全部作者: 张坚,马琳,李先宽,李兴林,谭世强
作者单位: 天津中医药大学中药学院;天津科技大学生物工程学院;天津中医药大学实验教学部
摘 要: 目的:考察诱导子茉莉酸甲酯(methyl jasmonic acid,MeJA)对杠柳不定根中主要次生产物杠柳毒苷和4-甲氧基水杨醛生物合成的影响,并探讨其诱导过程中的生理机制。方法:培养杠柳不定根,并在杠柳不定根生长稳定期(第20天)中加入10,100,200 mmol/L的MeJA,诱导3 d后收获,测量次生产物杠柳毒苷和4-甲氧基水杨醛含量以及生理指标。结果:在MeJA浓度为10 mmol/L时,杠柳毒苷和4-甲氧基水杨醛的诱导合成能力最强(分别为空白对照组的2.75倍和2.26倍)。生理指标中脯氨酸、可溶性糖、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)、过氧化氢酶(catalase,CAT)随MeJA浓度的增加先增加后减少,而丙二醛(malondialdehyde,MDA)一直增加。结论:低浓度的MeJA可有效促进杠柳不定根中杠柳毒苷和4-甲氧基水杨醛的积累,MeJA作为诱导子可开启植物自身保护机制,清除自由基,增加抗性。
关 键 词: 中药资源学;杠柳不定根;茉莉酸甲酯;生理机制;杠柳毒苷;4-甲氧基水杨醛
Title: Studies on MeJA inducing effect on important secondary metabolites in Periploca sepium Bunge adventitious roots and on plant physiological mechanism
Author: ZHANG Jian, MA Lin, LI Xiankuan, LI Xinglin, TAN Shiqiang
Organization: School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine; College of Biotechnology, Tianjin University of Science and Technology; Department of Experiment Education, Tianjin University of Traditional Chinese Medicine
Abstract: Objective: To study the inducing effect of methyl jasmonic acid (MeJA) on Periploca sepium Bunge adventitious roots in the biosynthesis of the secondary product periplocin and 4-methoxy salicylaldehyde, and to discuss the physiological mechanism in the process of induction. Methods: Culture P. sepium Bunge adventitious roots and add MeJA with 10, 100, 200 mmol/L into the cultures at the stable stage (the 20th day). The cultures were induced for 3 d and then the adventitious roots were harvested and measured with physiological index and the content of periplocin and 4-methoxy salicylaldehyde. Results: The induced synthesis of periplocin and 4-methoxy salicylaldehyde was the strongest when the concentration of MeJA was 10 mmol/L (2.75 times and 2.26 times compared with control group, respectively). The contents of proline, soluble sugar, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) first increased and then decreased with the increase of the concentration of MeJA, while the content of malondialdehyde (MDA) increased. Conclusion: Low concentration of MeJA can effectively promote the accumulation of periplocin and 4-methoxy salicylaldehyde in P. sepium Bunge adventitious roots. MeJA, a kind of elicitor, can stimulate the plant’s protection mechanism, clear free radicals and increase resistance of the cultures.
Key words: resource science of traditional Chinese medicine; Periploca sepium Bunge adventitious root; methyl jasmonic acid; physiological mechanism; periplocin; 4-methoxy salicylaldehyde
发表期数: 2017年3月第5期
引用格式: 张坚,马琳,李先宽,等. 茉莉酸甲酯对杠柳不定根中次生产物的诱导和生理机制研究[J]. 中国科技论文在线精品论文,2017,10(5):540-547.
 
0 评论数 0
暂无评论
友情链接