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麦冬多糖MDG-1改善脂质代谢紊乱作用机制研究

发表时间:2017-03-15  浏览量:1029  下载量:379
全部作者: 王旭,石林林,冯怡
作者单位: 上海中医药大学中药现代制剂技术教育部工程研究中心
摘 要: 目的:通过膳食诱导肥胖动物模型研究麦冬多糖MDG-1改善脂质代谢紊乱的作用机制。方法:32只雄性C57BL/6小鼠按体重随机分为正常低脂(low-fat diet,LFD)组、高脂(high-fat diet,HFD)组、MDG-1干预(HFD+MDG-1)组及能量限制(calorie restriction,CR)组,实验持续12周,实验记录体重、摄食量、基础体温,分离血清测定脂质代谢紊乱关键性指标,分离脂肪组织用于组织切片及实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)分析。结果:与HFD组相比,MDG-1可有效控制小鼠体重增加,升高基础体温,改善血脂水平,减少脂肪细胞大小,增加褐脂肪线粒体解偶联蛋白(uncoupling protein,UCP)基因的表达。结论:麦冬多糖MDG-1可以改善肥胖小鼠脂质代谢紊乱,其作用机制可能与增加褐脂肪产热基因表达、提高小鼠基础代谢率有关。
关 键 词: 中药药理学;麦冬多糖;脂质代谢紊乱;基础体温;线粒体解偶联蛋白
Title: Hypolipidemic effects of MDG-1, a polysaccharide derived from Ophiopogon japonicas, in a diet induced obesity mouse model of lipid metabolism disorders
Author: WANG Xu, SHI Linlin, FENG Yi
Organization: Engineering Research Center of Modern Preparation Technology of TCM, Ministry of Education, Shanghai University of Traditional Chinese Medicine
Abstract: Objective: This research focused on the hypolipidemic effects of MDG-1, a polysaccharide derived from Ophiopogon japonicas, in a diet induced obesity mouse model of lipid metabolism disorders and the underlying mechanism. Methods: 32 male C57BL/6 mice were treated with low-fat diet (LFD), high-fat diet (HFD), high-fat mixed MDG-1 diet (HFD+MDG-1) and a calorie restriction (CR) diet for 12 weeks. Body weight and food intakes were tested every day while basal body temperature was measured at 12 weeks. At the end of the experiment, blood samples were collected for biochemical examination, and adipose tissues were collected for histological examination and quantitative real-time PCR (qRT-PCR) analysis. Results: Compared with HFD, MDG-1 could control weight gain and increase body temperature. In addition, MDG-1 could ameliorate plasma lipid profiles, attenuate the size of adipocyte and increase the expression of mitochondrial uncoupling protein (UCP) genes in brown fat. Conclusion: MDG-1 increases energy expenditure by affecting the expression of some mitochondrial UCP related genes, thus inducing its action against hyperlipidemia.
Key words: pharmacology of traditional Chinese medicine; Ophiopogon polysaccharide; lipid metabolism disorders; basal body temperature; mitochondrial uncoupling protein
发表期数: 2017年3月第5期
引用格式: 王旭,石林林,冯怡. 麦冬多糖MDG-1改善脂质代谢紊乱作用机制研究[J]. 中国科技论文在线精品论文,2017,10(5):548-556.
 
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