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远志挥发油成分提取工艺及GC-MS分析

发表时间:2017-03-15  浏览量:1189  下载量:231
全部作者: 马媛,张琳,丁越佳,高怡,张悦,张晨新
作者单位: 大连医科大学中西医结合学院(研究院)
摘 要: 目的:优选远志挥发油的提取工艺,对远志化学成分进行分析,为评价其药用价值提供依据。方法:采用水蒸气蒸馏法提取远志挥发油,正交试验设计法考察加水量、浸泡时间、蒸馏时间和粉碎度对提取工艺的影响,优选最佳提取工艺;利用气相色谱-质谱联用(gas chromatography-mass spectrography,GC-MS)分析挥发油成分,通过峰面积归一化法测定各成分的相对质量分数。结果:远志挥发油最佳提取工艺为粉碎度10目,加水量为药量的7倍量,浸泡时间0 h,提取时间5 h;共鉴定出16种化学成分,并确定其相对含量。结论:远志挥发油中鉴定出的16种成分总含量达到100%,其中顺式十八碳烯酸含量占62.67%,其次为正十六烷酸,占17.43%. 本研究为远志挥发油提取工艺的确定提供科学依据。
关 键 词: 中药学;远志;挥发油;气相色谱-质谱联用;最佳提取工艺
Title: Extraction process and GC-MS analysis of volatile oil constituents of Radix Polygalae
Author: MA Yuan, ZHANG Lin, DING Yuejia, GAO Yi, ZHANG Yue, ZHANG Chenxin
Organization: College (Institute) of Integrative Medicine, Dalian Medical University
Abstract: Objective: To study the best extraction process of the volatile oil from Radix Polygalae, and analyze the chemical constituents, which provided a reference for evaluating its medicinal value. Methods: Water steam distillation method was applied to extract the volatile oil, the amount of water, soaking time, distillation time and crushing degree were optimized by orthogonal test. Chemical constituents in the volatile oil were analyzed by gas chromatography-mass spectrography (GC-MS), the relative mass fraction of each constituent was determined by peak area normalization method. Results: The optimal water-extraction process was as follows: crushing degree was 10 mesh, the amount of water was 7 times of the traditional Chinese drug, no soaking time, the extraction time was 5 h. 16 compounds were identified and their relative contents were determined. Conclusion: The total content of 16 compounds identified from the volatile oil of Radix Polygalae was 100%, among them cis-vaccenic acid was the major chemical constituent in the volatile oil which accounting for 62.67%, following was the n-hexadecanoic acid which accounting for 17.43%. Meanwhile, a scientific basis was provided for determining the extraction process of the volatile oil of Radix Polygalae.
Key words: science of Chinese pharmacology; Radix Polygalae; volatile oil; gas chromatography-mass spectrography; optimal extraction process
发表期数: 2017年3月第5期
引用格式: 马媛,张琳,丁越佳,等. 远志挥发油成分提取工艺及GC-MS分析[J]. 中国科技论文在线精品论文,2017,10(5):557-561.
 
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