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代谢工程大肠杆菌离子液体预处理竹水解液

发表时间:2017-05-15  浏览量:1257  下载量:154
全部作者: 赵德周,郑杰,王丹
作者单位: 重庆大学化学化工学院
摘 要: 作为一种原料,丁二酸被广泛应用于合成多种商品和特种化学品。通过构建大肠杆菌突变体DW103[在大肠杆菌突变体DC1515中共同过表达磷酸烯醇式丙酮酸羧化酶(phosphoenolpyruvate carboxylase,ppc)基因和碳酸酐酶(cyanobacterial carbonic anhydrase,ecaA)基因]可生产0.967 g丁二酸/g葡萄糖。利用与竹水解产物类似的含有葡萄糖、木糖和阿拉伯糖比例(质量比)为0.6:0.3:0.1的模拟糖混合物为底物,DW103可生产12.36 g/L丁二酸,产量为0.824 g/g总糖。在3-L生物反应器中,以离子液体预处理的竹水解产物为底物,代谢工程菌DW103可生产48.32 g/L丁二酸,产量为1.06 g/g总还原糖。本研究首先报道了以离子液体预处理的竹水解产物为底物,通过代谢工程大肠杆菌生产丁二酸,不仅扩大了竹子的应用领域,同时有助于降低微生物合成丁二酸的成本。
关 键 词: 发酵工程;丁二酸;离子液体;竹水解产物;大肠杆菌;发酵
Title: Production research on succinic acid from ionic liquid-pretreated bamboo hydrolysate by metabolically engineered Escherichia coli strain
Author: ZHAO Dezhou, ZHENG Jie, WANG Dan
Organization: School of Chemistry and Chemical Engineering, Chongqing University
Abstract: Succinic acid is widely used as a feedstock for the synthesis of a number of commodity and specialty chemicals. In this study, Escherichia coli mutant DW103 was constructed by overexpressing phosphoenolpyruvate carboxylase (ppc) and cyanobacterial carbonic anhydrase (ecaA) genes together in E. coli mutant DC1515. The engineered strain DW103 could produce 0.967 g succinic acid/g glucose. On a modeled sugar mixture containing glucose, xylose and arabinose with a ratio of 0.6:0.3:0.1 similar to that of the bamboo hydrolysate, DW103 produced 12.36 g/L succinic acid and the yield was 0.824 g/g total sugar. In a 3-L bioreactor with ionic liquid-pertreated bamboo enzymatic hydrolysate, the production of succinic acid by DW103 was about 48.32 g/L and the yield was 1.06 g/g total reducing sugars. This work firstly reported that succinic acid could be produced from ionic liquid-pretreated bamboo hydrolysate by metabolically engineered E. coli, which expanded the application field of bamboo and contributed to the decrease of the process cost of the microbial synthesis of succinic acid.
Key words: fermentation engineering; succinic acid; ionic liquid; bamboo hydrolysate; Escherichia coli; fermentation
发表期数: 2017年5月第9期
引用格式: 赵德周,郑杰,王丹. 代谢工程大肠杆菌离子液体预处理竹水解液[J]. 中国科技论文在线精品论文,2017,10(9):954-963.
 
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