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基于28S rDNA序列对10种蝇蛹寄生蜂的分类及系统发育研究

发表时间:2017-05-15  浏览量:1442  下载量:333
全部作者: 胡泽章,孙猛,吕兵,翟一凡,陈浩,郑礼,于毅
作者单位: 山东省农业科学院植物保护研究所
摘 要: 本研究利用28S rDNA基因片段为分子标记,采用DNA 条形码分析方法研究10种蝇蛹寄生蜂的分类鉴定技术,旨在探究该分子标记鉴定蝇蛹寄生蜂的可行性,以期建立一种快速、准确鉴定蝇蛹寄生蜂的新方法。结果显示,扩增的28S rDNA序列长665 bp,A+T含量均为41.5%;碱基转换与颠换的比值为1.43,序列的保守性较强。从系统发育树的聚类分析来看,每种蝇蛹寄生蜂均能形成单独分支。从种间和种内遗传距离来看,28S rDNA序列平均种间遗传距离是种内的64.67倍,该分子标记均能很好地对蝇蛹寄生蜂进行分类鉴定。
关 键 词: 农业昆虫与害虫防治;蝇蛹寄生蜂;28S rDNA基因;分子鉴定;分子系统发育
Title: Classification and phylogenetic study of 10 species of housefly pupal parasitoids based on 28S rDNA gene sequences
Author: HU Zezhang, SUN Meng, LÜ Bing, ZHAI Yifan, CHEN Hao, ZHENG Li, YU Yi
Organization: Institute of Plant Protection, Shandong Academy of Agricultural Sciences
Abstract: In this study, by using 28S rDNA gene as molecular marker, we aimed to make classification and molecular identification analysis of 10 common species of housefly pupal parasitoids with DNA bar coding technology. We explored the feasibility of molecular marker identification of housefly pupal parasitoids in order to establish a new method for rapid and accurate identification of housefly pupal parasitions. The results are as follows. The average length of 28S rDNA sequences was 665 bp. A+T content was about 41.5%; the ratio of base transition to transversion was 1.43, the sequences were relatively conservative. Each pupal parasitoid formed a separate branch according to the cluster analysis of the phylogenetic tree. The interspecific genetic distance was 64.67 times of the intraspecific genetic distance of sequence of 28S rDNA. 28S rDNA gene is effective molecular markers to identify species of housefly pupal parasitoids.
Key words: agricultural entomology and pest control; housefly pupal parasitoid; 28S rDNA gene; molecular identification; molecular phylogeny
发表期数: 2017年5月第9期
引用格式: 胡泽章,孙猛,吕兵,等. 基于28S rDNA序列对10种蝇蛹寄生蜂的分类及系统发育研究[J]. 中国科技论文在线精品论文,2017,10(9):964-971.
 
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