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HGF通过激活ERK信号通路减弱TGF-β1的促纤维化作用

发表时间:2017-06-15  浏览量:125  下载量:17
全部作者: 韩沛林,崔清波,范美丽,陆文君,李洲,李昭铸
作者单位: 哈尔滨医科大学第二临床医学院小儿外科
摘 要: 目的:探究肝细胞生长因子(hepatocyte growth factor,HGF)降低转化生长因子-β1(transforming growth factor-β1,TGF-β1)诱导纤维化的作用和可能机制。方法:使用不同浓度的HGF对体外培养的大鼠成纤维细胞进行预处理,随后进行TGF-β1的纤维化诱导,检测诱导后肌腱成纤维细胞的纤维连接蛋白(fibronectin)及α-平滑肌肌动蛋白(alpha smooth muscle actin,α-SMA)的表达及细胞外调节蛋白激酶(extracelluar regulated protein kinase,ERK)磷酸化情况。随后阻断ERK信号通路,再次观察fibronectin及α-SMA的表达情况。结果:HGF可以减弱TGF-β1诱导纤维化的作用。当阻断ERK信号通路时,这种中和作用明显减弱。结论:本实验证实HGF可通过ERK信号通路减弱TGF-β1对肌腱成纤维细胞的纤维化作用。
关 键 词: 外科学;肝细胞生长因子;转化生长因子-β1;纤维化
Title: HGF attenuating the fibrosis of TGF-β1 by activating ERK signaling pathway
Author: HAN Peilin, CUI Qingbo, FAN Meili, LU Wenjun, LI Zhou, LI Zhaozhu
Organization: Pediatric Department, The Second Affiliated Hospital of Harbin Medical University
Abstract: Objective: We explore the role of hepatocyte growth factor (HGF) in reducing transforming growth factor-β1 (TGF-β1)-induced fibrosis and the possible mechanism. Methods: The fibroblasts cultured in vitro were pretreated with different concentrations of HGF. The fibrosis induction of TGF-β1 was performed and the expression of fibronectin and α-smooth muscle actin (α-SMA) in the induced tendon fibroblasts and the phosphorylation of extracelluar regulated protein kinase (ERK) were detected. Followed by blocking the ERK signaling pathway, then we observed fibronectin and α-SMA expression. Results: HGF can attenuate the effect of TGF-β1 on fibrosis, and this neutralization is significantly attenuated when the ERK signaling pathway is blocked. Conclusion: This study confirms that HGF can reduce the fibrosis of TGF-β1 on tendon fibroblasts by ERK signaling pathway.
Key words: surgery; hepatocyte growth factor; transforming growth factor-β1; fibrosis
发表期数: 2017年6月第11期
引用格式: 韩沛林,崔清波,范美丽,等. HGF通过激活ERK信号通路减弱TGF-β1的促纤维化作用[J]. 中国科技论文在线精品论文,2017,10(11):1196-1202.
 
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