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自噬在马凡综合征小鼠主动脉组织中的表达及作用机制
发表时间：2017-06-15 浏览量：1845 下载量：629
| 全部作者： | 彭群勇，张赛丹，张雯瑄，彭振宇 |
| 作者单位： | 中南大学湘雅医院心血管内科；中南大学湘雅二医院急诊科 |
| 摘 要： | 目的：通过建立马凡综合征小鼠模型，检测马凡综合征小鼠主动脉中的自噬水平并探讨其作用机制。方法：将6只雌性野生型C57BL/6J小鼠作为对照组，6只雌性杂合子型FBN1C1039G/+小鼠作为马凡综合征组，饲养8个月后处死小鼠，收集主动脉标本，通过对主动脉直径、HE染色进行病理分析，透射电子显微镜（transmission electron microscope，TEM）检测小鼠主动脉中自噬小体的数量，Western blotting检测小鼠主动脉中LC3-II、p62的蛋白表达水平。结果：与对照组比较，马凡综合征组小鼠主动脉的直径及弹性纤维断裂的程度均显著增加；TEM结果显示马凡综合征组小鼠主动脉中自噬小体的数量较对照组显著减少；与对照组比较，马凡综合征组小鼠主动脉中LC3-II的蛋白表达显著降低，而p62的蛋白表达显著增加。结论：自噬在马凡综合征小鼠主动脉中显著降低，其可能参与抑制p62介导的蛋白降解。 |
| 关 键 词： | 心血管病学；马凡综合征；主动脉瘤；平滑肌细胞；自噬 |
| Title： | Expression of autophagy in aortic tissues in a mouse model of Marfan syndrome and its underlying mechanism |
| Author： | PENG Qunyong, ZHANG Saidan, ZHANG Wenxuan, PENG Zhenyu |
| Organization： | Department of Cardiovascular Medicine, Xiangya Hospital, Central South University; Department of Emergency, The Second Xiangya Hospital of Central South University |
| Abstract： | Objective: To investigate the expression of autophagy in aortic tissues in the established mouse model of Marfan syndrome and explore its mechanism. Methods: A total of 6 female wild-type C57BL/6J mice were divided into the control group and 6 female heterozygotes FBN1C1039G/+ mice were divided into the Marfan syndrome group. The mice were fed for 8 months and sacrificed. The aortic tissues were collected for further experiments. The pathological analysis was conducted by detecting aorta diameter and HE staining, the number of aortic autophagosomes was counted by transmission electron microscope (TEM), and the protein expressions of LC3-II and p62 were detected by Western blotting. Results: Both the diameter of aorta and the degree of elastic fiber rupture increased more significantly in the Marfan syndrome group than that of the control group. TEM results indicated that the number of autophagosomes was obviously decreased in the aorta smooth muscle cells of the Marfan syndrome group compared with the control group. In addition, the protein expression of LC3-II in the Marfan syndrome group was obviously decreased compared with the control group, while the protein expression of p62 was significantly increased in the Marfan syndrome group. Conclusion: The autophagy was significantly decreased in the aortic tissues of the Marfan syndrome group, which might contribute to decreasing the degradation of protein mediated by p62. |
| Key words： | cardiovascular diseases; Marfan syndrome; aortic aneurysm; smooth muscle cells; autophagy |
| 发表期数： | 2017年6月第11期 |
| 引用格式： | 彭群勇，张赛丹，张雯瑄，等. 自噬在马凡综合征小鼠主动脉组织中的表达及作用机制[J]. 中国科技论文在线精品论文，2017，10（11）：1210-1215. |
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