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不同金霉素盐对小鼠免疫器官指数和血液指标的影响

发表时间:2017-06-15  浏览量:107  下载量:33
全部作者: 刘灯,王建华
作者单位: 重庆大学生物工程学院
摘 要: 在日粮中分别添加150 mg/kg金霉素(chlortetracycline,CTC)盐酸盐(CTC•HCl)、金霉素硫酸盐(CTC•H2SO4)和金霉素甲磺酸盐(CTC•CH3SO3H)饲喂的昆明小鼠为实验组,正常饲喂小鼠为对照组,分别于21 d和42 d测定小鼠胸腺和脾脏指数,检测血液中CTC及免疫球蛋白IgA、IgM和IgG的含量。实验结果表明:1)饲喂21 d后,CTC•H2SO4和CTC•CH3SO3H组胸腺指数显著高于对照组(P<0.05),CTC•HCl组无显著变化(P>0.05),且随着饲喂时间的继续延长胸腺指数均降低;CTC•HCl、CTC•H2SO4和CTC•CH3SO3H组脾脏指数与对照组比较,均无统计学意义(P>0.05)。2)CTC•H2SO4和CTC•CH3SO3H组小鼠血清中CTC的含量显著高于CTC•HCl组(P<0.01)。3)CTC•HCl、CTC•H2SO4和CTC•CH3SO3H组IgA含量显著低于对照组(P<0.05),并且随着饲喂时间的延长,持续抑制了IgA的形成;IgM在21 d时受到抑制(P<0.01),但在42 d时,CTC•H2SO4和CTC•CH3SO3H组却显著升高(P<0.01),说明CTC对IgM的形成先抑制后促进;在21 d和42 d,实验组中IgG含量与对照组比较均无显著差别(P>0.05)。因此,可以推断CTC∙H2SO4和CTC•CH3SO3H也可用于动物饲料添加剂,并可拓展CTC的靶向动物、提高CTC的生物利用度。
关 键 词: 药效学;金霉素;免疫器官指数;血液指标
Title: Effect of different chlortetracycline salts on immune organ index and serum index of mouse
Author: LIU Deng, WANG Jianhua
Organization: Bioengineering College, Chongqing University
Abstract: Chlortetracycline hydrochloride (CTC∙HCl), chlortetracycline bisulfate (CTC∙H2SO4) and chlortetracycline mesylate (CTC∙CH3SO3H) were added daily to feed the mice as the treatment groups, and the control group did not receive any treatment. After raised 21 d and 42 d, the thymus index and spleen index were measured. The concentrations of CTC and the level of IgA, IgM and IgG in serum were also detected. The results are as followed: 1) Thymus indexes of the CTC∙H2SO4 and CTC∙CH3SO3H treatment groups were higher than control group (P<0.05), but there was no significant difference between CTC∙HCl group and control group (P>0.05). Thymus indexes of all groups were reduced at 42 d, and there was no clear difference on spleen index (P>0.05). 2) The serum CTC concentrations of CTC∙H2SO4 and CTC∙CH3SO3H groups were significantly higher than CTC∙HCl group (P<0.01). 3) The concentrations of IgA in CTC∙HCl, CTC∙H2SO4 and CTC∙CH3SO3H groups were lower than control group both at 21 d and 42 d (P<0.05). The concentrations of IgM in treatment groups were lower than control group at 21 d (P<0.01). Interestingly, the CTC∙H2SO4 and CTC∙CH3SO3H groups showed significant increase of IgM concentration compared with control group and CTC∙HCl group at 42 d consecutively (P<0.01). As for IgG, all treatment groups had no significant difference with the control group at either 21 d or 42 d (P>0.05). As a consequence, the CTC∙H2SO4 and CTC∙CH3SO3H have the potential to be used as feed supplement, and could increase the bioavailability of CTC in more animals.
Key words: pharmacodynatics; chlortetracycline; immune organ index; serum index
发表期数: 2017年6月第11期
引用格式: 刘灯,王建华. 不同金霉素盐对小鼠免疫器官指数和血液指标的影响[J]. 中国科技论文在线精品论文,2017,10(11):1231-1236.
 
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