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壳聚糖包裹EGCG载药微球及其生物应用研究

发表时间:2017-07-13  浏览量:79  下载量:34
全部作者: 刘颖异,刘丽炜,胡思怡,王玥,岳婕,秦沛,黄昱霖,梁耀天
作者单位: 长春理工大学理学院
摘 要: 对壳聚糖(chitosan,CS)和表没食子儿茶素没食子酸酯((-)-epigallocatechin gallate,EGCG)构成的载药纳米微球的生物应用进行实验研究。首先制备了壳聚糖与EGCG偶联的载药纳米微球,通过琼脂糖凝胶电泳实验证明了两者的成功偶联。然后在磷酸盐缓冲溶液(phosphate buffer saline,PBS)环境下对其胶体稳定性进行了101 h监测,结果显示在101 h内该载药纳米微球无明显聚集、沉淀、降解;动态光散射粒径监测结果显示粒径大小保持稳定,证明其具有良好的生物稳定性。最后进行体内和体外生物应用研究,将制备的载药纳米微球用于小鼠乳腺癌肿瘤模型,在实验中壳聚糖包载表没食子儿茶素没食子酸酯(CS-EGCG)载药纳米微球注射组的小鼠肿瘤尺寸减小,表现出对体内MCF-7乳腺癌肿瘤的良好抑制效果,且抑制效果明显优于壳聚糖注射组与EGCG注射组。并在此基础上对其进行功能化修饰,实现了载药微球对MCF-7乳腺癌细胞的靶向荧光标记作用。载药纳米微球在生物医药领域显示出广泛的应用前景。
关 键 词: 光学;载药纳米微球;抑制;肿瘤
Title: Chitosan encapsulated EGCG loaded microspheres and its biological application study
Author: LIU Yingyi, LIU Liwei, HU Siyi, WANG Yue, YUE Jie, QIN Pei, HUANG Yulin, LIANG Yaotian
Organization: School of Science, Changchun University of Science and Technology
Abstract: In this paper, the biological applications of drug loaded nanospheres composed of chitosan (CS) and (-)-epigallocatechin gallate (EGCG) were studied. Firstly, the drug loaded nanospheres were prepared by coupling chitosan with EGCG, and the successful coupling was proved by agar gel electrophoresis. The colloidal stability were monitored in the phosphate buffer saline (PBS) environment in 101 h, the results showed that the microspheres had no obvious aggregation, precipitation and degradation in 101 h, and the dynamic light scattering particle size monitoring results showed that the particle sizes remained stable, which indicated CS-EGCG nanospheres have good biological stability. Finally, we conducted a research in the biological application of in vivo and in vitro, in which the preparated nanospheres were used in mouse breast cancer model. The tumor size of CS-EGCG nanospheres injection group was decreased and showed good inhibitory effect on MCF-7 breast cancer, and the inhibition effect was better than chitosan injection group and EGCG injection group. On this basis, the functional modification was carried out to achieve the targeted fluorescent labeling effect of drug loaded microspheres on MCF-7 breast cancer cells. Drug loaded nanospheres show wide application prospects in the biomedical field.
Key words: optics; drug loaded nanospheres; inhibition; tumor
发表期数: 2017年7月第13期
引用格式: 刘颖异,刘丽炜,胡思怡,等. 壳聚糖包裹EGCG载药微球及其生物应用研究[J]. 中国科技论文在线精品论文,2017,10(13):1493-1498.
 
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