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植物乳杆菌ZJ316的酵母凝集性及自身表面特性研究

发表时间:2017-08-15  浏览量:29  下载量:17
全部作者: 刘娜娜,王净净,顾青,郦萍
作者单位: 浙江工商大学食品与生物工程学院,浙江省食品微生物技术研究重点实验室
摘 要: 以本实验室保存的从婴儿粪便中筛选得到的一株产细菌素的植物乳杆菌(Lactobacillus plantarum)ZJ316为研究对象,用MATH(microbial adhesion to hydrocarbons)方法测定3株乳酸菌[植物乳杆菌ZJ316、植物乳杆菌ZJ22、屎肠球菌(Enterococcus faecium)ZJ19]的疏水性、表面电荷和自聚合能力等表面性质,并进行酵母凝集实验测定乳酸菌的糖类专一性,从而为更好地研究乳酸菌的粘附机制奠定良好的基础。结果显示,植物乳杆菌ZJ316的疏水性为 (83.67±1.13)%. 酵母凝集实验表明,植物乳杆菌ZJ316的酵母凝集度为32,加入了D-甘露糖和甲基-α-D-甘露糖苷的实验组,乳酸菌的粘附力下降,说明植物乳杆菌ZJ316的粘附与特异性粘附中的甘露糖结合相关联。
关 键 词: 食品生物化学;植物乳杆菌;黏附机制;酵母凝集性;表面性质;糖类专一性
Title: Study on yeast agglutination and surface characteristics of Lactobacillus plantarum ZJ316
Author: LIU Nana, WANG Jingjing, GU Qing, LI Ping
Organization: Zhejiang Provincial Key Laboratory of Food Microbiology, School of Food and Biotechnology, Zhejiang Gongshang University
Abstract: In this study, Lactobacillus plantarum ZJ316 is preserved in our laboratory, which is isolated from infant fecal samples and produce bacteriocin. We took the microbial adhesive to hydrocarbon (MATH) method to survey and evaluate the surface properties (hydrophobicity, surface charge and autopolymerization ability) of the three lactic acid bacteria including L. plantarum ZJ316, L. plantarum ZJ22, Enterococcus faecium ZJ19. The yeast agglutination experiment was used to make clear of lactic acid bacteria carbohydrate specificity. Therefore, it lays a good foundation for the study of the adhesion mechanism of lactic acid bacteria. The results showed that the value of hydrophobicity of L. plantarum ZJ316 is (83.67±1.13)%. Yeast agglutination experiments showed that the value of yeast agglutination for L. plantarum ZJ316 was 32. When the D-mannose and methyl alpha-D-mannose glucoside were added into the experimental group, the ability of yeast agglutination declined.It illustrated that the adhesion of L. plantarum ZJ316 was associated with mannose in specific adhesion.
Key words: food biochemistry; Lactobacillus plantarum; adhesion mechanism; yeast agglutination; surface property; carbohydrate specificity
发表期数: 2017年8月第15期
引用格式: 刘娜娜,王净净,顾青,等. 植物乳杆菌ZJ316的酵母凝集性及自身表面特性研究[J]. 中国科技论文在线精品论文,2017,10(15):1753-1759.
 
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