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HPLC同时测定三黄片中盐酸小檗碱、黄芩苷和大黄素的含量

发表时间:2017-09-15  浏览量:744  下载量:121
全部作者: 李映,屠鹏飞,王金铃
作者单位: 北京中医药大学中药学院
摘 要: 目的:建立利用高效液相色谱(high performance liquid chromatography,HPLC)法同时测定盐酸小檗碱、黄芩苷和大黄素含量的方法并用于测定三黄片中三种主要药效成分的含量。方法:采用Agilent C18(4.6 mm × 250 mm,5 μm)色谱柱,流动相为乙腈-0.5%磷酸溶液(三乙胺调pH=4.0),梯度洗脱,流速1 mL•min-1,检测波长275 nm. 结果:盐酸小檗碱、黄芩苷的线性范围为1~30 μg∙mL-1,大黄素的线性范围为0.5~15 μg∙mL-1,方法回收率为95.83%~100.09%. 结论:该方法简便、准确、重复性好,定量限低并且能排除其他成分的干扰,可用于三黄片质量控制的评价。
关 键 词: 药效学;三黄片;小檗碱;黄芩苷;大黄素;高效液相色谱
Title: Simultaneous determination of berberine hydrochloride, baicalin and emodin in Sanhuang tablets by HPLC
Author: LI Ying, TU Pengfei, WANG Jinling
Organization: School of Chinese Materia Medica, Beijing University of Chinese Medicine
Abstract: Objective: A rapid and specific high performance liquid chromatography (HPLC) method was developed for the analysis of berberine hydrochloride, baicalin and emodin. The method was applied to study the content determination of Sanhuang tablets containing berberine hydrochloride, baicalin and emodin. Methods: The analysis was carried out on a Agilent C18 reversed-phase column (4.6 mm × 250 mm, 5 μm) by gradient elution with acetonitrile and 0.5% phosphoric acid (adjusted pH=4.0 with triethylamine). The flow rate was 1 mL•min-1 and the detection wavelength was set at 275 nm. Results: Linear responses were obtained for berberine hydrochloride and baicalin ranging from 1 to 30 μg∙mL-1, emodin ranging from 0.5 to 15 μg∙mL-1. The extraction recovery ranged from 95.83% to 100.09%. Conclusion: This method is simple, accurate with good repeatability, low detection limit and can eliminate the interference of other components. This validated method could be used in the quality control of Sanhuang tablets.
Key words: pharmacodynatics; Sanhuang tablets; berberine hydrochloride; baicalin; emodin; high performance liquid chromatography
发表期数: 2017年9月第17期
引用格式: 李映,屠鹏飞,王金铃. HPLC同时测定三黄片中盐酸小檗碱、黄芩苷和大黄素的含量[J]. 中国科技论文在线精品论文,2017,10(17):1972-1976.
 
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