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柴芪汤对非酒精性脂肪肝大鼠肝脏组织氧化应激的影响及意义

发表时间:2017-09-15  浏览量:760  下载量:118
全部作者: 陈丽如,张立平,张嘉琰,彭龙
作者单位: 北京中医药大学东方医院消化科
摘 要: 目的:观察柴芪汤对非酒精性脂肪肝(non-alcoholic fatty liver disease,NAFLD)大鼠氧化应激的影响,并分析其对肝脏组织的保护作用。方法:将32只SPF级雄性SD大鼠随机分为正常组、模型组、柴芪汤组和罗格列酮组,除正常组外用高盐高脂高糖饮食喂养8周,用药组从造模第一天开始给予柴芪汤(5.67 g•kg-1•d-1)、罗格列酮混悬液(3 mg•kg-1•d-1)灌胃干预,8周后分别测定各实验组血清丙氨酸转氨酶(alanine aminotransferase,ALT)、天冬氨酸转氨酶(aspartate aminotransferase,AST)以及肝脏组织中的超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)及甘油三酯(triglyceride,TG)水平;光学显微镜观察肝组织脂肪病变程度。结果:模型组大鼠血清ALT、AST水平及肝脏组织MDA、TG水平明显高于正常组(P<0.05),SOD、GSH-Px水平明显低于正常组(P<0.05);用药组各项指标均低于模型组,差异有统计学意义(P<0.05);柴芪汤组大鼠ALT、AST水平较罗格列酮组无明显差异(P>0.05);柴芪汤组与罗格列酮组比较可明显升高肝脏组织内SOD及GSH-Px水平,有统计学差异(P<0.05)。结论:柴芪汤在调节NAFLD大鼠肝功能及血脂的水平上与罗格列酮作用效果相似,但在对抗氧化应激方面的效果优于罗格列酮,柴芪汤对大鼠NAFLD有一定的防治作用。
关 键 词: 中医内科学;非酒精性脂肪肝;柴芪汤;肝脏保护;氧化应激
Title: Effects of Chaiqi decoction on oxidative stress in the liver tissue from NAFLD rats
Author: CHEN Liru, ZHANG Liping, ZHANG Jiayan, PENG Long
Organization: Digestive Department, Dongfang Hospital, Beijing University of Chinese Medicine
Abstract: Objective: To observe the effects of Chaiqi decoction on oxidative stress in the non-alcoholic fatty liver disease (NAFLD) rat model, and analyze its protection for liver tissue. Methods: 32 rats were randomly divided into normal group, model group, Chaiqi decoction group and rosiglitazone group. The rat model was established by feeding high fat, high sugar and high salt diet for 8 weeks. The Chaiqi decoction group was given Chaiqi decoction (5.67 g•kg-1•d-1) from the beginning of modeling, and rosiglitazone group was given rosiglitazone (3 mg•kg-1•d-1). The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) in serum and the levels of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), triglyceride (TG) in liver tissue were detected after 8 weeks. Liver pathological histopathology among the 4 groups were observed under optical microscope. Results: In comparing with normal group, the levels of ALT, AST in serum and the levels of MDA, TG in liver tissue were significantly increased, while the levels of SOD and GSH-Px in liver tissue were decreased in model group (P<0.05). All the indexes of the two treatment groups were lower than model group, the difference was statistically significant (P<0.05). There was no significant difference between Chaiqi decoction group and rosiglitazone group in the levels of ALT and AST (P>0.05), but the levels of SOD and GSH-Px in liver tissue in Chaiqi decoction group were higher than those of rosiglitazone group. The difference was statistically significant (P<0.05). Conclusion: Chaiqi decoction and rosiglitazone both have positive therapeutic effects against hyperlipidemia and abnormal liver function in experimental NAFLD rats, better than rosiglitazone in oxidative stress.
Key words: internal medicine of traditional Chinese medicine; non-alcoholic fatty liver disease; Chaiqi decoction; liver protection; oxidative stress
发表期数: 2017年9月第17期
引用格式: 陈丽如,张立平,张嘉琰,等. 柴芪汤对非酒精性脂肪肝大鼠肝脏组织氧化应激的影响及意义[J]. 中国科技论文在线精品论文,2017,10(17):1977-1982.
 
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