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烯丙基荧光素−丙烯酰胺沉淀共聚荧光分子印迹选择性荧光检测三氟氯氰菊酯残留

发表时间:2017-10-13  浏览量:1803  下载量:274
全部作者: 高林,郑旭东,刘恩秀,刘恩利,李春香
作者单位: 江苏大学化学化工学院
摘 要: 设计并使用沉淀聚合法合成一种新颖的用于检测蜂蜜中超痕量三氟氯氰菊酯(lambda-cyhalothrin,LC)的荧光分子印迹聚合物(fluorescent molecularly imprinted polymer,FMIP)微球。以三氟氯氰菊酯为模板分子、丙烯酰胺(AM)为功能单体、烯丙基荧光素为荧光单体、二甲基丙烯酸乙二醇酯(EGDMA)为交联剂,合成微米尺寸具有特异识别位点的FMIP微球。FMIP微球展现出规则的几何构型、强烈的荧光发光特性以及对目标分子的高度选择识别性。而且FMIP微球的荧光强度与三氟氯氰菊酯的浓度呈良好的线性关系,由此建立了线性回归方程(I0/I)−1=0.29·CC−0.006 08,R2 = 0.993 6,检出限(limit of detection,LOD)= 0.004 nmol/L;实际样品检测结果证明,该方法制备的FMIP微球可以在复杂样品中有选择性地检测三氟氯氰菊酯浓度;再生实验证明FMIP微球至少可以重复利用5次且无明显的信号损失。
关 键 词: 分析化学;荧光传感器;三氟氯氰菊酯;选择性
Title: Selective detection of lambda-cyhalothrin residues by fluorescence molecular imprinting with precipitation polymerisation of allyl fluorescein and acrylamide
Author: GAO Lin, ZHENG Xudong, LIU Enxiu, LIU Enli, LI Chunxiang
Organization: School of Chemistry and Chemical Engineering, Jiangsu University
Abstract: A novel fluorescent molecularly imprinted polymer (FMIP) microspheres are fabricated via precipitation polymerisation, and used for detecting uitratrace amounts of lambda-cyhalothrin (LC) in honey. Using LC as template molecule, acrylamide (AM) as functional monomer, allyl fluorescein as fluorescent monomer, and ethylene glycol dimethacrylate (EGDMA) as crosslinker, the FMIP microspheres are prepared to form recognition sites with micrometer. The as-synthesized microspheres exhibit regular geometry, which possess high fluorescence intensity and highly selective recognition for target molecule. Moreover, the fluorescence intensity of FMIP microspheres show a good linear relationship with the concentration of LC, which are described by the Stern-Volmer equation with a correlation coefficient of 0.993 6. A lower limit of detection (LOD) is found to be 0.004 nmol/L. The results of practical detection suggest that FMIP microspheres by the developed method are satisfactory for selective determination of LC concentration in honey samples. The FMIP microspheres has certified that they could be reused for no less than 5 times without remarkably loss of signal intensity.
Key words: analytical chemistry; fluorescent sensor; lambda-cyhalothrin; selectivity
发表期数: 2017年10月第19期
引用格式: 高林,郑旭东,刘恩秀,等. 烯丙基荧光素−丙烯酰胺沉淀共聚荧光分子印迹选择性荧光检测三氟氯氰菊酯残留[J]. 中国科技论文在线精品论文,2017,10(19):2125-2131.
 
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