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胜红蓟黄脉病病原的分子鉴定

发表时间:2017-11-15  浏览量:1255  下载量:147
全部作者: 陈家盛,胡汝检,朱重庆,林文武,林文忠,林丹,张洁
作者单位: 福建农林大学植物保护学院,植物病毒研究所,福建省植物病毒学重点实验室
摘 要: 对福建农林大学校内表现黄花叶、明脉等疑似感染双生病毒症状的胜红蓟进行病原鉴定。利用双生病毒特异性简并引物PA/PB扩增得到500 bp的DNA-A部分片段,再根据已获得的片段设计特异性引物将其全长剩余部分约2.3 kb扩增出来,序列拼接得到全长2 734 nts. 序列分析发现,该病毒与台湾一种胜红蓟黄脉病毒的同源性高达99.5%. 同时,利用引物β01/β02扩增出DNA-β卫星的全长(1 346 nts)。序列分析发现,此卫星分子与一种胜红蓟黄脉病毒伴随的卫星分子的同源性最高,达 98.1%. 因此,判断此种病毒为胜红蓟黄脉病毒的一种分离物。
关 键 词: 植物病毒学;双生病毒;胜红蓟;DNA-A;DNA-β
Title: Molecular identification of the pathogen inducing Ageratum yellow vein disease
Author: CHEN Jiasheng, HU Rujian, ZHU Chongqing, LIN Wenwu, LIN Wenzhong, LIN Dan, ZHANG Jie
Organization: Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, College of Plant Protection, Fujian Agriculture and Forestry University
Abstract: In this study, Ageratum samples showing obvious yellow mosaic leaves and vein clearing geminiviral-like symptoms were selected in campus of Fujian Agriculture and Forestry University. By using the degenerate primers PA/PB for geminivirus, a partial DNA-A sequence with 500 bp was amplified. Then, using designed specific primers, the left sequence (around 2.3 kb) of the full-length was obtained. Sequence splicing showed the full length of 2 734 nts. Sequence analysis showed that this virus had a high sequence identity of 99.5% with Ageratum yellow vein virus isolated from Taiwan. Meanwhile, using primers β01/β02 for the betasatellite, the full-length of DNA-β was amplified, which was 1 346 nts. Sequence analysis showed that this betasatellite had a high sequence identity of 98.1% with an Ageratum yellow vein virus betasatellite. Therefore, the virus identified in this study was considered as an isolate of Ageratum yellow vein virus.
Key words: plant virology; geminivirus; Ageratum conyzoides; DNA-A; DNA-β
发表期数: 2017年11月第21期
引用格式: 陈家盛,胡汝检,朱重庆,等. 胜红蓟黄脉病病原的分子鉴定[J]. 中国科技论文在线精品论文,2017,10(21):2398-2403.
 
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