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滋补脾阴法对DACD大鼠海马线粒体功能障碍的作用研究

发表时间:2017-12-15  浏览量:1286  下载量:134
全部作者: 孙铮,战丽彬
作者单位: 大连医科大学中西医结合研究所;南京中医药大学基础医学院
摘 要: 目的:探讨滋补脾阴法调节糖尿病认知功能障碍(diabetes-associated cognitive decline,DACD)大鼠海马线粒体功能的机制。方法:将SD大鼠随机分为对照组(Con)、糖尿病组(Model)、滋补脾阴方药治疗组(ZBPYR)、补中益气汤治疗组(BZYQ)和六味地黄汤治疗组(LWDH)5组。通过2’,7’-二氯荧光黄双乙酸盐(DCFH-DA)荧光标记和JC-1染色法分别检测DACD大鼠海马活性氧(reactive oxygen species,ROS)含量、线粒体膜电位。Western blotting观察各组小鼠海马组织中细胞色素C(Cyto C)在胞核/胞浆中表达的差异。结果:1)ZBPYR组线粒体ROS含量较Model组降低(P<0.05),BZYQ和LWDH组与Model组相比无明显统计学差异(P>0.05);2)与Model组比较,ZBPYR组、BZYQ组海马组织线粒体膜电位明显升高(P<0.05),LWDH组线粒体膜电位变化不明显(P>0.05);3)ZBPYR组胞浆中Cyto C蛋白表达水平较Model组减弱(P<0.01),BZYQ和LWDH组中Cyto C蛋白表达水平较Model组无统计学意义(P>0.05);4)ZBPYR组、BZYQ和LWDH组胞核中Cyto C蛋白表达水平较Model组增强(P<0.01)。结论:滋补脾阴方药较补中益气汤、六味地黄汤更能明显改善DACD大鼠海马线粒体ROS含量、线粒体膜电位,并阻止线粒体中Cyto C向胞浆释放。推测滋补脾阴方药提高大鼠认知功能是通过调节线粒体功能完成的。结果提示,滋补脾阴法对糖尿病大鼠认知功能障碍的疗效优于健脾益气法和滋补肾阴法。
关 键 词: 中西医结合医学;滋补脾阴法;健脾益气法;滋补肾阴法;糖尿病认知功能障碍;线粒体功能
Title: Effects of Zibupiyin recipe on the mitochondrial dysfunction in the hippocampus of DACD rats
Author: SUN Zheng, ZHAN Libin
Organization: College (Institute) of Integrative Medicine, Dalian Medical University; College of Basic Medicine, Nanjing University of Chinese Medicine
Abstract: Objective: To investigate the mechanism of Zibupiyin recipe on the hippocampus mitochondria function of diabetes-associated cognitive decline (DACD) rats. Methods: SD rats were randomly divided into five groups: control group (Con), diabetes group (Model), Zibupiyin group (ZBPYR), Buzhongyiqi group (BZYQ) and Liuweidihuang group (LWDH). DCFH-DA fluorescence labeling and JC-1 staining were used to test the reactive oxygen species (ROS) level and mitochondrial membrane potential in hippocampus of DACD rats. Western blotting analysis was used to observe the expression difference of cytochrome C (Cyto C) in nucleus and cytosol. Results: 1) The mitochondrial ROS level of the ZBPYR group was decreased when compared with the Model group (P<0.05), while the BZYQ and LWDH groups had no significance (P>0.05). 2) The levels of mitochondrial membrane potential in the ZBPYR and BZYQ groups were increased when compared with the Model group (P<0.05), while the LWDH group had no significance (P>0.05). 3) The expression level of Cyto C in cytosol of the ZBPYR group was significantly lower than the Model group (P<0.01), while the BZYQ and LWDH groups had no significance (P>0.05). 4) The expression levels of Cyto C in nucleus of the ZBPYR, BZYQ and LWDH groups were significantly higher than the Model groups (P<0.01). Conclusion: The improvement in the levels of ROS and the mitochondrial membrane potential, and the inhibition of the release of Cyto C to cytosol after ZBPYR treatment are more obvious than BZYQ and LWDH groups. These results might be associated with the function of mitochondrial. By taking the effect of the test, we further make sure that the curative effect of Zibupiyin recipe on cognitive dysfunction in diabetic rats is better than Jianpiyiqi recipe and Zibushenyin recipe.
Key words: Chinese and western integrative medicine; Zibupiyin recipe; Jianpiyiqi recipe; Zibushenyin recipe; diabetes-associated cognitive decline; mitochondrial function
发表期数: 2017年12月第23期
引用格式: 孙铮,战丽彬. 滋补脾阴法对DACD大鼠海马线粒体功能障碍的作用研究[J]. 中国科技论文在线精品论文,2017,10(23):2655-2660.
 
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