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针刺调节焦虑模型大鼠下丘脑C型利钠肽受体的机制研究

发表时间:2017-12-15  浏览量:1305  下载量:274
全部作者: 姚弘,魏大能,蔡定均,余曙光,张承舜,吕鹏,彭晓华,周奇志
作者单位: 乐山市中医医院针灸康复科;成都中医药大学针灸推拿学院
摘 要: 目的:运用神经化学技术激活中枢下丘脑室旁核C型利钠肽(C-type natriuretic peptide,CNP)的受体——利钠肽受体B(natriuretic peptide receptor B,NPR-B),明确下丘脑NPR-B在针刺抗焦虑中的作用。方法:将健康清洁级SD雄性大鼠随机分为6组,即空白组、模型组、针刺组、侧脑室注射生理盐水组(假注射组)、侧脑室注射NPR-B激动剂组(模药组)、侧脑室注射NPR-B激动剂针刺组(针药组)。建立慢性不可预知情绪应激(chronic unpredictable stress,CUS)焦虑模型。针刺组和针药组选取具有“宁心安神”功效的“内关”、“神门”穴进行针刺治疗。运用高架十字迷宫(elevated plus maze,EPM)测试各组大鼠的焦虑行为。采用免疫组化(immunohistochemistry,IHC)法检测大鼠下丘脑室旁核CNP与其受体NPR-B,以及下丘脑室旁核促肾上腺皮质激素释放激素(corticotropin-releasing hormone,CRH)的表达。采用酶联免疫吸附测定(enzyme linked immunosorbent assay,ELISA)法检测血浆皮质酮(corticosterone,CORT)水平。结果:1)行为学OT%值:模型组较空白组显著降低(P<0.01),针刺组明显高于模型组,假注射组、模药组、针药组明显低于针刺组(P<0.01),针药组与模药组比较无统计学差异(P>0.05)。2)下丘脑室旁核CNP和NPR-B:与空白组相比,模型组、假注射组、模药组、针药组CNP和NPR-B平均光密度值均明显升高(P<0.01);针刺组较模型组CNP和NPR-B平均光密度值显著降低(P<0.01);模药组和针药组较假注射组CNP和NPR-B平均光密度值均有升高趋势,针药组与模药组比较无统计学意义(P>0.05)。3)下丘脑室旁核CRH:模型组、假注射组、模药组、针药组均较空白组明显升高(P<0.05),针刺组相比模型组降低明显(P<0.05);模药组、针药组较假注射组均有升高,且针药组升高明显(P<0.05)。4)血浆CORT水平:模型组、模药组较空白组明显升高(P<0.01),针刺组与模型组相比明显降低(P<0.01),针药组较模药组有所降低,但未显示统计学差异(P>0.05)。结论:针刺“宁心安神”治疗焦虑障碍的作用可能与调节下丘脑CNP及其受体、抑制下丘脑-垂体-肾上腺(hypothalamic-pituitary-adrenal,HPA)轴的活性有关。下丘脑室旁核NPR-B激动剂具有能够削弱针刺的抗焦虑效应和调节HPA轴的作用,提示中枢下丘脑室旁核NPR-B可能是针刺发挥抗焦虑效应的关键环节之一。
关 键 词: 针灸学;焦虑模型大鼠;下丘脑室旁核;C型利钠肽;利钠肽受体B;下丘脑-垂体-肾上腺(HPA)轴
Title: Mechanism of acupuncture on C-type natriuretic peptide receptor in hypothalamus of anxiety model rats
Author: YAO Hong, WEI Daneng, CAI Dingjun, YU Shuguang, ZHANG Chengshun, LÜ Peng, PENG Xiaohua, ZHOU Qizhi
Organization: Department of Acupuncture Rehabilitation, Leshan City Hospital of Traditional Chinese Medicine; College of Acupuncture and Massage, Chengdu University of Traditional Chinese Medicine
Abstract: Objective: Using neurochemical techniques to activate the receptor of C-type natriuretic peptide (CNP), natriuretic peptide receptor B (NPR-B), in the central hypothalamic paraventricular nucleus, and to determine the role of hypothalamic NPR-B in anti-anxiety with acupuncture. Methods: Healthy and clean SD male rats were randomly divided into 6 groups, namely blank group, model group, acupuncture group, intracerebroventricular injection of saline group (sham injection group), intracerebroventricular injection of NPR-B agonist group (model drug group), intracerebroventricular injection of NPR-B agonist acupuncture group (acupuncture drug group). Anxiety models were established by chronic unpredictable stress (CUS). The acupuncture group and acupuncture drug group were treated by acupuncture at the Neiguan point and Shenmen point which had the effect of calming the mind and calming the nerves. Anxiety behavior tests were conducted using elevated plus maze (EPM) test in rats of each group. The expressions of CNP and its receptor NPR-B, and the corticotropin-releasing hormone (CRH) in the hypothalamic paraventricular nucleus were detected by immunohistochemistry (IHC) method. The level of plasma corticosterone (CORT) was determined by enzyme linked immunosorbent assay (ELISA). Results: 1) Behavior OT%: The model group was significantly lower than the blank group (P<0.01). The acupuncture group was significantly higher than the model group. The sham injection group, model drug group and acupuncture drug group were significantly lower than the acupuncture group (P<0.01). There was no statistical difference between the acupuncture drug group and model drug group (P>0.05). 2) CNP and NPR-B in the hypothalamic paraventricular nucleus: Compared with the blank group, the average optical density of CNP and NPR-B in the model group, sham injection group, model drug group and acupuncture drug group significantly increased (P<0.01). Compared with the model group, the average optical densities of CNP and NPR-B decreased significantly in the acupuncture group (P<0.01). Compared with the sham injection group, both the model drug group and acupuncture drug group had a higher tendency of the average optical density, but there is no statistical significance between the acupuncture drug group and model drug group (P>0.05). 3) Hypothalamic paraventricular nucleus CRH: The model group, sham injection group, model drug group and acupuncture drug group were significantly higher than the blank group (P<0.05), but compared with the model group, the acupuncture group decreased significantly (P<0.05). Compared with the sham injection group, the model drug group and acupuncture drug group increased, and the acupuncture drug group increased significantly (P<0.05). 4) Plasma CORT: The model group and model drug group were significantly higher than the blank group (P<0.01). Compared with the model group, the acupuncture group decreased obviously (P<0.01). Compared with the model drug group, the acupuncture drug group decreased, but there was no statistical difference (P>0.05). Conclusion: The effect of acupuncture on the treatment of anxiety disorders may be related to the regulation of hypothalamus CNP and its receptor and the activity of inhibiting hypothalamic-pituitary-adrenal (HPA) axis. The NPR-B agonist in the hypothalamic paraventricular nucleus can weaken the anti-anxiety effect of acupuncture and regulate the role of the HPA axis, suggesting that the central hypothalamic paraventricular nucleus NPR-B may be one of the key links in which acupuncture plays an anti-anxiety effect.
Key words: science of acupuncture and moxibustion; anxiety model rats; hypothalamic paraventricular nucleus; C-type natriuretic peptide; natriuretic peptide receptor B; hypothalamic-pituitary-adrenal (HPA) axis
发表期数: 2017年12月第23期
引用格式: 姚弘,魏大能,蔡定均,等. 针刺调节焦虑模型大鼠下丘脑C型利钠肽受体的机制研究[J]. 中国科技论文在线精品论文,2017,10(23):2661-2670.
 
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