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基于Fe3O4-Au 磁性纳米粒子的双酚A电化学发光适配体传感器研究

发表时间:2017-12-29  浏览量:1364  下载量:153
全部作者: 姜梦凡,康天放
作者单位: 北京工业大学环境与能源工程学院
摘 要: 制备核壳型Fe3O4@SiO2@Au复合物。以实验室自制的磁性玻碳电极(magnetic glassy carbon electrode,MGCE)为基础电极,在其表面修饰Fe3O4@SiO2@Au. 使氨基修饰的双酚A(bisphenol A,BPA)适配体(aptamer,Apt)与巯基修饰的互补DNA(cDNA)链发生杂交反应,生成双链DNA(dsDNA). 制备羧基功能化的CdS量子点(quantum dots,QDs),使dsDNA中适配体的氨基与CdS QDs的羧基形成共价键,制得dsDNA-CdS. 将该dsDNA-CdS通过形成Au—S化学键连接到上述电极表面,制成了双酚A 适配体传感器(记作MGCE/Fe3O4@SiO2@Au-dsDNA-CdS)。双酚A与适配体结合后会从电极表面脱落,与之相连的CdS QDs也会随之脱离电极表面,电化学发光(electrochemiluminescence,ECL)信号减弱。该双酚A适配体传感器的ECL信号与双酚A浓度在1.0×10−10~1.0×10−5 mol/L范围内呈现良好的线性关系,检出限为8×10−11 mol/L. 实验结果表明,该适配体传感器对双酚A 响应的选择性好,灵敏度高。
关 键 词: 环境化学;双酚A;核酸适配体;磁性电极;CdS 量子点
Title: Study on electrochemiluminescence aptamer sensor for bisphenol A based on Fe3O4-Au magnetic nanoparticles
Author: JIANG Mengfan, KANG Tianfang
Organization: College of Environmental and Energy Engineering, Beijing University of Technology
Abstract: In this paper, we prepared core shell composites of Fe3O4@SiO2@Au nanoparticles. The laboratory self-made magnetic glassy carbon electrodes (MGCE) were used as basic electrodes and Fe3O4@SiO2@Au composites were modified onto the surface of the electrodes. Double stranded DNA(dsDNA) was synthesized by hybridization between amino modified bisphenol A (BPA) aptamers (Apt) and thiol modified complementary DNA (cDNA) chain. CdS quantum dots (QDs) functionalized with carboxyl groups were prepared. The covalent bonds were formed between the amino group of Apt in dsDNA and the carboxyl group of CdS QDs to prepare dsDNA-CdS. The dsDNA-CdS was modified the surface of above-mentioned electrode by forming Au—S chemical bond to prepare bisphenol A aptamer sensor (denoted as MGCE/Fe3O4@SiO2@Au-dsDNA-CdS). After being bound with bisphenol A, the Apt and the connected CdS QDs would fall off from the electrode surface, and the electrochemiluminescence (ECL) signals would weaken. ECL signals of bisphenol A aptamer sensor showed a good linear relationship with bisphenol A concentration in the range of 1.0×10−10-1.0×10−5 mol/L, with a detection limit of 8×10−11 mol/L. The experimental results indicated that the aptamer sensor for bisphenol A possessed both good selectivity and high sensitivity.
Key words: environmental chemistry; bisphenol A; nucleic acid aptamer; magnetic electrodes; CdS quantum dots
发表期数: 2017年12月第24期
引用格式: 姜梦凡,康天放. 基于Fe3O4-Au 磁性纳米粒子的双酚A电化学发光适配体传感器研究[J]. 中国科技论文在线精品论文,2017,10(24):2696-2706.
 
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