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复方丹参有效组分及其组合物对MIF诱导人神经母细胞瘤细胞SH-SY5Y细胞凋亡的作用
发表时间：2018-03-15 浏览量：1519 下载量：234
| 全部作者： | 梁承杰，曾水云，魏婷，陈晨，杨洁 |
| 作者单位： | 南京大学生命科学学院医药生物技术国家重点实验室；军事医学科学院放射与辐射医学研究所蛋白质组学国家重点实验室 |
| 摘 要： | 为探究复方丹参（Fufang Danshen，FFDS）有效成分及其组合物对巨噬细胞移动抑制因子（macrophage migration inhibitory factor，MIF）诱导人神经母细胞瘤细胞SH-SY5Y细胞凋亡的作用机制，采用噻唑蓝（MTT）法检测细胞生长情况；利用流式细胞仪技术检测细胞凋亡；通过Western blotting法检测目标蛋白的表达。研究结果表明，MIF表现出与Aβ1-42相似的神经毒性，能够促进SH-SY5Y细胞的凋亡。MIF可通过诱导SH-SY5Y细胞中Bad的表达量增加来引起细胞凋亡。而复方丹参组合物、丹参酮IIA（tanshinone IIA，Tan IIA）和三七皂苷R1（notoginsenoside R1，NG-R1）能够显著抑制MIF诱导的细胞凋亡，并能明显减少Bad的表达量。进而推测，复方丹参组合物可能通过下调Bad的表达而发挥神经保护作用。 |
| 关 键 词： | 中药学；巨噬细胞移动抑制因子（MIF）；复方丹参有效组分组合物；细胞凋亡；促凋亡蛋白Bad；人神经母细胞瘤细胞 |
| Title： | Role of the effective components of Fufang Danshen and their composition in MIF-induced apoptosis in human neuroblastoma cells SH-SY5Y |
| Author： | LIANG Chengjie, ZENG Shuiyun, WEI Ting, CHEN Chen, YANG Jie |
| Organization： | State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University; State Key Laboratory of Protemics, Beijing Institute of Radiation, Academy of Military Medical Sciences |
| Abstract： | The objective of this study are explored the mechanism of effective components of Fufang Danshen (FFDS or Danshen compound) and their composition (FFDS’s composition) on macrophage migration inhibitory factor (MIF)-induced apoptosis in human neuroblastoma cells SH-SY5Y. Here, cell growth, apoptosis and target protein expression are detected by MTT assay, flow cytometry and Western blotting, respectively. The results show as follows: MIF presents neurotoxicity similar to Aβ1-42 and can promote apoptosis of SH-SY5Y cells. MIF can induce the high expression of Bad in SH-SY5Y cells and then trigger apoptosis. FFDS’s composition, tanshinone IIA (Tan IIA) and notoginsenoside R1 (NG-R1) can obviously inhibit apoptosis and reduce the expression of Bad in MIF-induced SH-SY5Y cells. Therefore, we speculate that FFDS’s composition might play a role in the protection of nerve through down-regulating the expression of Bad in neurodegenerative diseases. |
| Key words： | science of Chinese pharmacology; macrophage migration inhibitory factor (MIF); composition of effective components of Fufang Danshen; apoptosis; proapoptotic protein Bad; human neuroblastoma cell |
| 发表期数： | 2018年3月第5期 |
| 引用格式： | 梁承杰，曾水云，魏婷，等. 复方丹参有效组分及其组合物对MIF诱导人神经母细胞瘤细胞SH-SY5Y细胞凋亡的作用[J]. 中国科技论文在线精品论文，2018，11（5）：505-515. |
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