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野生烟草中TMV抗性基因的遗传定位

发表时间:2018-05-15  浏览量:233  下载量:42
全部作者: 吴竹筠,陈炯炯
作者单位: 华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室
摘 要: 烟草花叶病毒(tobacco mosaic virus,TMV)在烟草和蔬菜中危害较大,给生产带来了巨大损失。抗性品种的利用,将大大减少由TMV感染带来的损失。研究以TMV抗病基因型的二倍体野生烟草(Nicotiana paniculata)为材料,通过抗病基因型与感病基因型进行杂交,自交后获得F2代分离群体。根据表型鉴定,野生烟草中TMV抗性基因为单基因。开发分子标记筛选重组单株,最终初步定位了野生型烟草中的TMV 抗性基因候选基因所在区段在标记3.0M与标记N1之间。野生烟草中TMV抗性基因的定位,将为抗性育种提供参考信息。
关 键 词: 遗传学;遗传定位;烟草花叶病毒抗性基因;烟草
Title: Genetic mapping of the tobacco mosaic virus (TMV) resisitance gene in wild tobacco
Author: WU Zhujun, CHEN Jiongjiong
Organization: Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University
Abstract: The tobacco mosaic virus (TMV) is known to infect tobacco and vegetables, and it can cause serious losses in production. The best way to avoid losses in crops is to grow resistant varieties against TMV. In this study, we use a TMV resistant genotype to cross with a susceptible genotype from a wild tobacco Nicotiana paniculata which generates a F2 segregating population. According to the phenotypes of the population, the TMV resistance is controlled by a single gene. Using molecular marks to select recombinant individual, the candidate gene of TMV resistance genes in N. paniculata is mapped between marks 3.0M and N1. This information can be used in the resistance breeding.
Key words: genetics; genetic mapping; tobacco mosaic virus resistant gene; tobacco
发表期数: 2018年5月第9期
引用格式: 吴竹筠,陈炯炯. 野生烟草中TMV抗性基因的遗传定位[J]. 中国科技论文在线精品论文,2018,11(9):875-878.
 
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