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ghrelin 在有氧运动对高胆固醇血症小鼠心肌损伤 保护中的作用

发表时间:2018-05-15  浏览量:624  下载量:94
全部作者: 朱镕鑫,付鹏宇,龚丽景,杨贤罡
作者单位: 北京体育大学运动人体科学学院;北京体育大学中国运动与健康研究院;河北省体育科学研究所
摘 要: 目的:通过对高胆固醇血症小鼠的运动干预,探究有氧运动保护心肌组织的可能机制。方法:取刚离乳雄性C57BL/6J小鼠30只,随机分为普通对照组(NC,n=8)和高胆固醇血症建模组(H,n=22),其中H组饲喂高脂饲料(40 kcal%脂肪,1.25%胆固醇,0.5%胆酸)(1 kcal=4.184 kJ)。8周后建模成功的H 组小鼠被随机分为对照组(HC,n=7)和运动组(HE,n=7),其中HE组进行4 周有氧运动。苏木精-伊红(hematoxylin-eosin,HE)染色观察心肌组织形态学变化,测试血清中血糖、甘油三酯和总胆固醇水平;利用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)和Western blotting法分别检测心肌组织中ghrelin、Tnni3、Npy1r 及Vegfa 基因的mRNA表达和ghrelin、Ghsr-1a、Tnni3、GOAT、Npy1r及Vegfa蛋白的表达。结果:1)运动干预后,HE组体重增量显著小于HC组;2)形态学检查发现,HE组小鼠心肌梗死区域小于HC组;3)HE组小鼠血糖和甘油三酯较HC组均出现下降;4)HE组ghrelin mRNA表达较HC组显著上升,Tnni3及Vegfa mRNA表达较HC组上调;Npy1r mRNA表达较HC组出现下调;5)HE组ghrelin、Ghsr-1a较HC组蛋白表达显著上调,Tnni3 和Vegfa蛋白较HC组有上调趋势,GOAT、Npy1r蛋白表达较HC组出现下调。结论:有氧运动能够降低高胆固醇血症小鼠血清甘油三酯水平,修复心肌纤维损伤,ghrelin可能在其心肌损伤保护中起一定的作用,但其内部保护机制有待进一步研究。
关 键 词: 运动生物化学;高胆固醇血症;有氧运动;心肌损伤
Title: The role ghrelin played in hypercholesterolemia mice’s myocardial protection under aerobic exercise intervention
Author: ZHU Rongxin, FU Pengyu, GONG Lijing, YANG Xiangang
Organization: Sports Science College, Beijing Sports University, Beijing 100084,Sports Science College, Beijing Sports University, Beijing 100084,Sports Science Research Center, Beijing Sports University, Beijing 100084,Hebei Provincial Research Institute of Sports Science, Shijiazhuang 050011
Abstract: Objective: To explore the potential mechanism of myocardial tissue protection in hypercholesterolemia mice under the intervention of aerobic exercise. Methods: Thirty C57BL/6J weaning male mice were randomly divided into feed normal chow group (NC, n=8) and feed high cholesterol chow group (H, 40 kcal% fat, 1.25% cholesterol, 0.5% cholic acid, n=22) (1 kcal=4.184 kJ). After 8 weeks’ modeling, the mice with hypercholesterolemia were randomly and equally divided into HE group (n=7) and HC group (n=7). Mice in HE group were applied with aerobic exercise intervention for four weeks. The morphological changes of myocardial tissue were performed by hematoxylin-eosin (HE) staining. Serum samples were collected for the determination of glucose (GLUZ), triglyceride (TG) and total cholesterol (CHO). The mRNA expression levels of ghrelin, Tnni3, Npy1r and Vegfa in myocardium tissue were measured by quantitative real-time PCR (qRT-PCR), and protein expression levels of ghrelin, Ghsr-1a, Tnni3, GOAT, Npy1r and Vegfa were detected by Western blotting method. Results: Taking HC as a reference group to HE, after intervention, weight gained less and myocardial infarct reduced; GLUZ and TG decreased; ghrelin mRNA expression level increased significantly in HE with Tnni3 and Vegfa expression levels heightened, and Npy1r mRNA level decreased; the protein levels of ghrelin and Ghsr-1a in HE group up-regulated significantly. Tnni3 and Vegfa level went up in HE while GOAT level went slightly down. Npy1r protein expression decreased in HE. Conclusion: Aerobic exercise can improve hypercholesterolemia symptoms to some extent and ghrelin may play an important role in the protection of myocardial tissue, but its internal protection mechanism needs further research.
Key words: sports biochemistry; hypercholesterolemia; aerobic exercise; myocardial damage
发表期数: 2018年5月第9期
引用格式: 朱镕鑫,付鹏宇,龚丽景,等. ghrelin 在有氧运动对高胆固醇血症小鼠心肌损伤 保护中的作用[J]. 中国科技论文在线精品论文,2018,11(9):932-940.
 
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