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将军山全沟硬蜱体内伯氏疏螺旋体的分型

发表时间:2018-09-14  浏览量:132  下载量:29
全部作者: 刁沛文,宋春玲,王晓旭,吴婷婷,黄江,于佳颖,常巧呈
作者单位: 黑龙江八一农垦大学动物科技学院
摘 要: 为确定采集于哈尔滨将军山的全沟硬蜱体内是否携带伯氏疏螺旋体(Borrelia burgdorferi)及其分型情况,本研究应用巢式PCR方法对伯氏疏螺旋体进行检测,并进一步应用分型标记基因OspA和groEL进行分型。应用巢式PCR方法在全沟硬蜱体内检测到伯氏疏螺旋体,OspA和groEL的长度分别为287 bp和289 bp,进化分析结果显示,本实验的伯氏疏螺旋体均与GenBank登录的阿弗西尼疏螺旋体(B. afzelii)序列在同一进化分支上。研究结果表明,采集于哈尔滨将军山的全沟硬蜱携带伯氏疏螺旋体,类型为阿弗西尼疏螺旋体(B. afzelii)。
关 键 词: 医学寄生虫学;伯氏疏螺旋体分型;全沟硬蜱;将军山
Title: Typing of Borrelia burgdorferi in Ixodes persulcatus from General hill
Author: DIAO Peiwen, SONG Chunling, WANG Xiaoxu, WU Tingting, HUANG Jiang, YU Jiaying, CHANG Qiaocheng
Organization: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University
Abstract: In order to determine whether Borrelia burgdorferi is carried in Ixodes persulcatus collected from General hill in Harbin and to identify the type of B. burgdorferi, nested PCR was used to detect B. burgdorferi, and the typing marker genes OspA and groEL were further used for genotyping. B. burgdorferi was detected in I. persulcatus by nested PCR. The lengths of OspA and groEL were 287 bp and 289 bp, respectively. The evolutionary analysis results showed that the sequences of B. burgdorferi in this experiment were all on the same evolutionary branch as the sequences of B. afzelii registered in GenBank. The results indicated that the type of B. burgdorferi carried by I. persulcatus from General hill in Harbin was B. afzelii.
Key words: medical parasitology; typing of Borrelia burgdorferi; Ixodes persulcatus; General hill
发表期数: 2018年9月第17期
引用格式: 刁沛文,宋春玲,王晓旭,等. 将军山全沟硬蜱体内伯氏疏螺旋体的分型[J]. 中国科技论文在线精品论文,2018,11(17):1713-1718.
 
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