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基于PPI网络搜寻结直肠癌的中枢基因
发表时间：2019-08-30 浏览量：1769 下载量：342
| 全部作者： | 王亚楠，张娟 |
| 作者单位： | 华北电力大学数理学院 |
| 摘 要： | 目的：通过生物信息学方法基于蛋白互作（protein-protein interaction，PPI）网络搜寻结直肠癌的中枢基因。方法：为搜寻结直肠癌的中枢基因，分析了3个来自Gene Expression Omnibus（GEO）数据库的基因芯片数据集（GSE21815、GSE21510、GSE113513）。首先，将维恩分析得到的差异表达基因进行功能富集和通路分析，了解其聚类原因。然后，通过STRING数据库构建差异表达基因的PPI网络，并进行子网络模块分析，以更加精准地发掘结直肠癌发生过程的核心路径。最后，依据结合度筛选出结直肠癌发生过程中的中枢基因。结果：研究发现了在整个PPI网络中结合分数较高的前10个基因，将这10个基因确定为结直肠癌发生过程中起关键作用的中枢基因，并发现这些基因与细胞周期过程密切相关。结论：所发现的中枢基因可以成为结直肠癌的治疗靶点。 |
| 关 键 词： | 应用数学；结直肠癌；蛋白互作网络；中枢基因 |
| Title： | Searching for hub genes of colorectal cancer based on PPI network |
| Author： | WANG Yanan, ZHANG Juan |
| Organization： | School of Mathematics and Physics, North China Electric Power University |
| Abstract： | Objective: To search for hub genes of colorectal cancer based on protein-protein interaction (PPI) network through bioinformatics methods. Methods: To search for the hub genes of colorectal cancer, three gene chip datasets (GSE21815, GSE21510, GSE113513) from the Gene Expression Omnibus (GEO) database were analyzed. Firstly, the differentially expressed genes obtained by Venn analysis were subjected to functional enrichment and pathway analysis to understand the reason for clustering. Then, the PPI network of differentially expressed genes was constructed by STRING database, and sub-network module analysis was carried out to explore the core path of colorectal cancer more accurately. Finally, the hub genes were screened based on the degree of binding during colorectal carcinogenesis. Results: The top ten genes with higher binding scores were found in the entire PPI network. These ten genes were identified as hub genes that played a key role in the colorectal carcinogenesis, and these genes were closely related to the cell cycle process. Conclusion: The discovered hub genes can be the therapeutic target for colorectal cancer. |
| Key words： | applied mathematics; colorectal cancer; protein-protein interaction network; hub genes |
| 发表期数： | 2019年8月第4期 |
| 引用格式： | 王亚楠，张娟. 基于PPI网络搜寻结直肠癌的中枢基因[J]. 中国科技论文在线精品论文，2019，12（4）：579-588. |
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