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荧光假单胞菌中转录因子NodD2_7的功能研究

发表时间:2019-08-30  浏览量:153  下载量:6
全部作者: 张孟元,王勇,马海龙,何永兴,郭玎玎
作者单位: 兰州大学药学院;兰州大学生命科学学院
摘 要: 根瘤菌中的LysR家族转录因子NodD可以响应植物黄酮并调控相关基因。然而,研究发现荧光假单胞菌中该蛋白的同源蛋白NodD2_7并不能结合芹菜素、根皮素、槲皮素这三种典型的植物黄酮。构建nodD2_7基因敲除菌株并进行非标定量的全蛋白质组学实验,鉴定到1 590个可定量蛋白,其中140个发生显著变化。生物信息学分析表明,该转录因子影响的差异蛋白与多种生物学过程相关,包括次生代谢产物的合成与代谢、氨基酸的合成与代谢、细胞分裂、I型和VI型分泌系统、运动性、生物膜生成、趋化性、氧化应激等。基于质谱数据的表型验证显示,NodD2_7可以显著正调控荧光假单胞菌的运动能力。
关 键 词: 微生物学;NodD2_7 蛋白;蛋白质组学;运动性
Title: Study on the function of the transcription factor NodD2_7 from Pseudomonas fluorescens 2P24
Author: ZHANG Mengyuan, WANG Yong, MA Hailong, HE Yongxing, GUO Dingding
Organization: School of Pharmacy, Lanzhou University; School of Life Sciences, Lanzhou University
Abstract: It is reported that NodD in rhizobia, belongs to LysR-type transcription factor, can respond to plant flavonoids and mediate related genes. While, we found the homologous protein NodD2_7 of the NodD in Pseudomonas fluorescens 2P24 can not bind to three typical plant flavonoids, apigenin, phloretin and quercetin. In order to investigate the function of NodD2_7 in P. fluorescens 2P24, the gene knockout strains of nodD2_7 were constructed and a label-free quantitative proteomics experiment was carried out. Finally, 1 590 quantifiable proteins were identified, up to 140 of which changed significantly. Bioinformatics analysis show that, these differentially expressed proteins fall into many functional categories, including secondary metabolites synthesis and metabolism, amino acid synthesis and metabolism, cell division, type I and VI secretion system, motility, biofilm formation, chemotaxis, oxidative stress and so on. Phenotypic verification according to mass spectrometry data shows that NodD2_7 can significantly up-regulate the motility of P. fluorescens 2P24.
Key words: microbiology; protein NodD2_7; proteomics; motility
发表期数: 2019年8月第4期
引用格式: 张孟元,王勇,马海龙,等. 荧光假单胞菌中转录因子NodD2_7的功能研究[J]. 中国科技论文在线精品论文,2019,12(4):630-637.
 
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