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一种手持式POCT荧光检测系统设计
发表时间：2019-12-31 浏览量：778 下载量：83
| 全部作者： | 王慧泉，吴鸽，胡猛，赵喆，韩广，王金海 |
| 作者单位： | 天津工业大学电子与信息工程学院 |
| 摘 要： | 荧光技术广泛应用于医学检测。为对弱荧光信号进行及时有效的检测，本文研制了一种手持式即时检测（point-of-care testing，POCT）荧光检测系统。该设备使用32位STM32F103VET6嵌入式系统作为微控处理器，控制12位模数（analog-to-digital，A/D）转换器对光电传感器采集到的电信号进行采样和后续的信号处理。以9种标准浓度（10～50 mg/L）荧光溶液为检测对象，使用赛凡7-FRSpec系列荧光光谱测试系统作为对比仪器，进行分析实验。结果表明，在10～50 mg/L浓度范围内，本系统所测数据与荧光光谱系统所测数据拟合曲线的相关系数为0.924，预测集均方根误差为0.083 V，具有较好的应用前景。 |
| 关 键 词： | 信息处理技术；荧光技术；荧光检测；即时检测；相关系数 |
| Title： | Design of a handheld POCT fluorescence detection system |
| Author： | WANG Huiquan, WU Ge, HU Meng, ZHAO Zhe, HAN Guang, WANG Jinhai |
| Organization： | School of Electronics and Information Engineering, Tiangong University |
| Abstract： | Fluorescence technology is widely used in medical detection. In order to timely and effectively detect weak fluorescent signals, a handheld point-of-care testing (POCT) fluorescence detection system was developed in this paper. The device used a 32-bit STM32F103VET6 embedded system as a micro-controller, the 12-bit analog-to-digital (A/D) converter was controlled to sample the electrical signals collected by the photoelectric sensor and to perform subsequent signal processing. 9 kinds of standard concentration fluorescent solutions of 10-50 mg/L were used as test objects, in which Safran 7-FRSpec series fluorescence spectrum test system was utilized as a contrast instrument to conduct the analytical experiment. The results showed that the correlation coefficient between experimental data and fitting curve of data measured by fluorescence spectroscopy system was 0.924, and the root mean square error of prediction dataset was 0.083 V, which had good application prospects. |
| Key words： | information processing technology; fluorescence technology; fluorescence detection; point-of-care testing (POCT); correlation coefficient |
| 发表期数： | 2019年12月第6期 |
| 引用格式： | 王慧泉，吴鸽，胡猛，等. 一种手持式POCT荧光检测系统设计[J]. 中国科技论文在线精品论文，2019，12（6）：1022-1028. |
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