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利用RNA-Seq技术探究三法三穴对坐骨神经损伤大鼠的修复机制

发表时间:2020-10-10  浏览量:282  下载量:15
全部作者: 吕桃桃,于天源
作者单位: 北京中医药大学针灸推拿学院
摘 要: 目的:探究推拿对坐骨神经损伤(sciatic nerve injury,SNI)大鼠神经损伤点基因表达的影响,从转录组水平阐明推拿促进周围神经损伤的恢复机制。方法:27只SD大鼠随机分为假手术组、模型组和三法三穴组。三法三穴组大鼠在造模7 d后,每日以点法、拨法和揉法定性定量刺激大鼠术侧殷门穴、承山穴和阳陵泉穴1次,干预10次休息1 d,共干预20次。采用RNA测序(RNA-Seq)技术检测神经损伤点基因变化,并做GO(gene ontology)分析和KEGG(Kyoto encyclopedia of genes and genomes)分析。结果:假手术组与模型组比较,共计431个基因发生显著性差异变化;模型组与三法三穴组比较,共计221个基因发生显著性差异变化;假手术组与三法三穴组比较,共计322个基因发生显著性差异变化。结论:推拿促进周围神经损伤大鼠的恢复是通过改变神经损伤点基因序列实现的。
关 键 词: 中医学;推拿;坐骨神经损伤;RNA测序;神经损伤点;修复机制
Title: Using RNA-Seq technique to explore the repair mechanism of three methods and three points on rats with sciatic nerve injury
Author: LÜ Taotao, YU Tianyuan
Organization: School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine
Abstract: Objective: To explore the effect of massage on the gene expression of nerve injury point in rats with sciatic nerve injury (SNI), and to elucidate the mechanism of massage promoting the recovery of peripheral nerve injury at the transcriptome level. Methods: 27 SD rats were randomly divided into three groups: sham group, model group, three methods and three points group. In the three methods and three points group, after 7 d of modeling, the rats were stimulated at Yinmen, Chengshan and Yanglingquan on the operating side with point method, dial method and rubbing method once a day, 10 times for rest and one day for a total of 20 times. RNA sequencing (RNA-Seq) technique was used to detect the gene changes of nerve injury point, GO (gene ontology) analysis and KEGG (Kyoto encyclopedia of genes and genomes) analysis were performed. Results: Compared with the model group, 431 genes in the sham group changed significantly. Compared with the three methods and three points group, 221 genes in the model group changed significantly. Compared with the three methods and three points group, 322 genes in the sham group changed significantly. Conclusion: Massage can promote the recovery of rats with peripheral nerve injury by changing the gene sequence of nerve injury point.
Key words: traditional Chinese medicine; massage; sciatic nerve injury; RNA sequencing; nerve injury point; repair mechanism
发表期数: 2020年9月第3期
引用格式: 吕桃桃,于天源. 利用RNA-Seq技术探究三法三穴对坐骨神经损伤大鼠的修复机制[J]. 中国科技论文在线精品论文,2020,13(3):315-323.
 
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