您的位置：首页 > 论文页面
不同国家和地区SARS-CoV-2序列的密码子使用偏好性及聚类分析
发表时间：2021-09-30 浏览量：748 下载量：133
| 全部作者： | 刘建忠，郑智捷 |
| 作者单位： | 云南大学云南省软件工程重点实验室 |
| 摘 要： | 目的：研究8个国家和地区SARS-CoV-2序列的密码子使用偏好性和演化关系。方法：分别求出8个国家和地区SARS-CoV-2序列中的最大相似序列组的基准SARS-CoV-2序列，用CodonW软件分析基准SARS-CoV-2序列的密码子使用偏好，用MEGA-X软件分析各国家和地区基准SARS-CoV-2序列之间演化关系。结果：基准SARS-CoV-2序列的G+C含量均低于A+U含量，其中加拿大最低，中国次之；8个国家和地区的共有使用偏好性密码子有19个，不包括中国的有7个，不包括加拿大的有1个，不包括中国和加拿大的有1个，这总计28个密码子中有25个以A、U结尾；中国有特有偏好性密码子7个，其中6个以C、G结尾，加拿大有1个，以U结尾；3个发育树将基准SARS-CoV-2序列分成两大类，美国、印度、智利和比利时为一类，澳大利亚、加拿大、英格兰和中国为另一类，在大类中进一步划分时存在不确定性。结论：SARS-CoV-2为了适应不同宿主在不断地发生突变，与宿主发生了协同进化，呈现出多源传染、多路演化态势。 |
| 关 键 词： | 生物工程；SARS-CoV-2；基准SARS-CoV-2序列；密码子使用偏好性；聚类分析 |
| Title： | Codon usage bias and cluster analysis of SARS-CoV-2 sequences in different countries and territories |
| Author： | LIU Jianzhong, ZHENG Jeffrey |
| Organization： | Key Laboratory for Software Engineering of Yunnan Province, Yunnan University |
| Abstract： | Objective: The codon usage bias and evolution of the SARS-CoV-2 sequences in eight different countries and territories were studied. Methods: The datum SARS-CoV-2 sequences of the largest similar sequence group among SARS-CoV-2 sequences in eight different countries and territories were calculated, the codon usage bias of the datum SARS-CoV-2 sequences was analyzed using CodonW package, the evolutionary relationships between the datum SARS-CoV-2 sequences were analyzed using MEGA-X. Results: G+C contents of the SARS-CoV-2 sequences were all lower than A+U contents, and the lowest measure was observed in Canada, followed by China. There were 19 commonly owned preference codons in eight different countries and territories, and 7 in seven different countries and territories (excluding China), 1 in seven different countries and territories (excluding Canada), 1 in six different countries and territories (excluding China and Canada). 25 of these 28 codons ended with A and U. There were 7 proprietary preference codons in China, 6 of which ended with C or G, and 1 in Canada that ended with U. The datum SARS-CoV-2 sequences were divided into two major categories by three phylogenetic trees, with the datum SARS-CoV-2 sequences in the USA, India, Chile and Belgium as one category and the datum SARS-CoV-2 sequences in Australia, Canada, England and China as the other category. In further classification, there was uncertainty. Conclusion: In order to adapt to different hosts, SARS-CoV-2 was constantly mutating and co-evolving with the host, showing a trend of multi-source infection and multi-channel evolution. |
| Key words： | biotechnology; SARS-CoV-2; datum SARS-CoV-2 sequence; codon usage bias; cluster analysis |
| 发表期数： | 2021年9月第3期 |
| 引用格式： | 刘建忠，郑智捷. 不同国家和地区SARS-CoV-2序列的密码子使用偏好性及聚类分析[J]. 中国科技论文在线精品论文，2021，14（3）：314-323. |
2
评论数 0请您登录
暂无评论
下载全文
多维论文