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膜式微流控方法添加/去除低温保护剂的实验研究
发表时间：2016-12-15 浏览量：2080 下载量：661
| 全部作者： | 尹来山，周晓明，汪志琨，花友文，张莹 |
| 作者单位： | 电子科技大学机械电子工程学院；北京协和医学院&中国医学科学院输血研究所 |
| 摘 要： | 随着干细胞等材料在临床和科研中的广泛应用，低温保存面临着越来越多的微小体积对象，这就对低温保护剂（cryoprotectant agents，CPAs）的添加/去除工作提出了新的处理要求。研究提出一种基于微流控和膜分离技术的方法，并基于该方法建立了夹膜式微流控系统。实验证明，该方法可实现小体积样品细胞CPAs的平稳、可控添加/去除处理，有望大幅度提高小体积生物样品CPAs处理的效率和效果。 |
| 关 键 词： | 生物医学工程学；低温保护剂；微流控芯片；膜分离技术 |
| Title： | Experimental study of a membrane-based microfluidic approach for adding/removing cryoprotectant agents |
| Author： | YIN Laishan, ZHOU Xiaoming, WANG Zhikun, HUA Youwen, ZHANG Ying |
| Organization： | School of Mechatronics Engineering, University of Electronic Science and Technology of China; Institute of Blood Transfusion, Peking Union Medical College & Chinese Academy of Medical Sciences |
| Abstract： | Along with the wide application of stem cells and other materials in clinical and scientific researches, cryopreservation faces a growing number of small volume objects. Some new requirements have been raised during adding/removing cryoprotectant agents (CPAs) for such small volume objects. In this study, a novel membrane-based microfluidic approach was presented. Accordingly, a membrane-based microfluidic system was designed and fabricated by microfluidics and membrane separation technique. Experiment results demonstrated that the system can achieve stable and controllable adding/removing CPAs for small volume samples, and it will greatly improve the efficiency and effectiveness of adding/removing CPAs for small volume biological samples. |
| Key words： | biomedical engineering; cryoprotectant agents; microfluidic chip; membrane separation technique |
| 发表期数： | 2016年12月第23期 |
| 引用格式： | 尹来山，周晓明，汪志琨，等. 膜式微流控方法添加/去除低温保护剂的实验研究[J]. 中国科技论文在线精品论文，2016，9（23）：2426-2432. |
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