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微囊藻毒素对斑马鱼食欲调控基因的影响
发表时间：2017-08-15 浏览量：2237 下载量：441
| 全部作者： | 李广宇，李莉，方洁，钟兆山，薛理耀，何焱 |
| 作者单位： | 华中农业大学水产学院 |
| 摘 要： | 蓝藻水华大规模爆发时会产生大量有毒的微囊藻毒素（microcystin，MC），具有极大的危害。通过实验研究MC-LR对斑马鱼食欲的影响，选取不同剂量的MC-LR加入各组水体中，其中实验组的MC-LR终浓度依次为0.3，3，30 μg/L，对照组的MC-LR浓度为0. MC-LR暴露12周后采样，通过取样提取斑马鱼脑RNA，反转录为cDNA，利用实时荧光定量PCR（quantitative real-time PCR，qRT-PCR）检测食欲相关基因的转录变化。实验结果表明，当MC-LR浓度较低时，促食欲基因表达降低，MC-LR浓度升高后，促食欲基因表达升高，食欲会旺盛；同时也检测到，在MC-LR的存在下，抑制食欲基因表达降低，随后升高达到一个较稳定的状态。可见，MC-LR可通过干扰食欲相关基因影响鱼类的摄食。 |
| 关 键 词： | 水产学基础学科；微囊藻毒素；斑马鱼；促食欲基因；抑制食欲基因 |
| Title： | Effect of MC-LR on appetite-regulating genes in zebrafish |
| Author： | LI Guangyu, LI Li, FANG Jie, ZHONG Zhaoshan, XUE Liyao, HE Yan |
| Organization： | College of Fisheries, Huazhong Agricultural University |
| Abstract： | A large amount of microcystin (MC) would appear when large-scale blue-green algae breaks out, which has strong damages to environment. In this study, zebrafish were exposed to varied doses of MC-LR groups (0, 0.3, 3, 30 μg/L). After 12 weeks, RNA was extracted from the brain samples and reverse transcribed into cDNA. Fluorescence quantitative real-time PCR (qRT-PCR) reactions were performed to detect the expression of appetite-related genes and assess the changes of zebrafish appetite. The results showed that the expression of appetite-promoting genes decreased at a lower dose MC-LR group, while the expression of appetite-promoting genes increased at a higher dose MC-LR group. In addition, MC-LR could down-regulate the expression of appetite-inhibiting genes at a lower concentration, but it would up-regulate them in a higher concentration and reach to a stable state. MC-LR could disorder appetite-related genes, which might eventually interfere ingestion of zebrafish. |
| Key words： | basic subject of aquatic science; microcystin; zebrafish; appetite-promoting genes; appetite-inhibiting genes |
| 发表期数： | 2017年8月第15期 |
| 引用格式： | 李广宇，李莉，方洁，等. 微囊藻毒素对斑马鱼食欲调控基因的影响[J]. 中国科技论文在线精品论文，2017，10（15）：1738-1743. |
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